Highly-efficient synthesis of biogenic selenium nanoparticles by Bacillus paramycoides and their antibacterial and antioxidant activities

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Shuai He¹  Haiyu Long¹  Zhen Guo¹  Mengdi Liang¹  Hao Shi¹  Han Cheng¹  Zhengwei Liu¹  Pei Liu¹  Min Sun²  Zhenlian Han³ 

[1]Faculty of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, China

[2]Jiangsu Provincial Key Construction Laboratory of Probiotics Preparation, Huaiyin Institute of Technology, Huaian, China

[3]Huai’an Municipal Center for Disease Control and Prevention, Huaian, China

[4]Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, School of Life Science, Huaiyin Normal University, Huaian, China

关键词: selenium nanoparticles;    Bacillus paramycoides;    antibacterial activity;    antioxidant activity;    green synthesis;   

DOI  :  10.3389/fbioe.2023.1227619

 received in 2023-05-23, accepted in 2023-07-21,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: Bacillus species are known for their ability to produce nanoparticles with various potential applications.Methods: In this study, we present a facile approach for the green synthesis of selenium nanoparticles (Se NPs) using the biogenic selenate-reducing bacterium Bacillus paramycoides 24522. We optimized the growth conditions and sodium selenite reduction efficiency (SSRE) of B. paramycoides 24522 using a response surface approach.Results: Se NPs were synthesized by reducing selenite ions with B. paramycoides 24522 at 37 °C, pH 6, and 140 r/min, resulting in stable red-colored Se NPs and maximal SSRE (99.12%). The synthesized Se NPs demonstrated lethality against Staphylococcus aureus and Escherichia coli with MICs of 400 and 600 μg/mL, and MBCs of 600 and 800 μg/mL, respectively, indicating the potential of Se NPs as antibacterial agents. Furthermore, the Se NPs showed promising antioxidant capabilities through scavenging DPPH radicals and reducing power.Discussion: This study highlights the environmentally friendly production of Se NPs using B. paramycoides 24522 and their possible applications in addressing selenium pollution, as well as in the fields of environment and biotechnology.

【 授权许可】

Unknown   
Copyright © 2023 Liu, Long, Cheng, Liang, Liu, Han, Guo, Shi, Sun and He.