Evolution of T cell receptor beta loci in salmonids

Frontiers in Immunology

Immunology

Unni Grimholt¹  Marie-Paule Lefranc²  Samuel Novas³  Susana Magadán³  Stanislas Mondot⁴  Pierre Boudinot⁵  Luc Jouneau⁵ 

[1] Fish Health Research Section, Norwegian Veterinary Institute, Oslo, Norway;IMGT, The International ImMunoGeneTics Information System® (IMGT), Laboratoire d´ImmunoGénétique Moléculaire (LIGM), Institut de Génétique Humaine (IGH), Centre National de la Recherche Scientifique (CNRS), University of Montpellier, Montpellier, France;Immunology Laboratory, Research Center for Nanomaterials and Biomedicine (CINBIO), Universidade de Vigo, Vigo, Spain;Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France;Université Paris-Saclay, INRAE, UVSQ, VIM, Jouy-en-Josas, France;

关键词: T cell receptor;    salmonid fish;    repertoire;    TRB locus;    adaptive immunity;    evolution;    rainbow trout;   

DOI  :  10.3389/fimmu.2023.1238321

 received in 2023-06-11, accepted in 2023-07-17,  发布年份 2023

来源: Frontiers

【 摘 要 】

T-cell mediated immunity relies on a vast array of antigen specific T cell receptors (TR). Characterizing the structure of TR loci is essential to study the diversity and composition of T cell responses in vertebrate species. The lack of good-quality genome assemblies, and the difficulty to perform a reliably mapping of multiple highly similar TR sequences, have hindered the study of these loci in non-model organisms. High-quality genome assemblies are now available for the two main genera of Salmonids, Salmo and Oncorhynchus. We present here a full description and annotation of the TRB loci located on chromosomes 19 and 25 of rainbow trout (Oncorhynchus mykiss). To get insight about variations of the structure and composition of TRB locus across salmonids, we compared rainbow trout TRB loci with other salmonid species and confirmed that the basic structure of salmonid TRB locus is a double set of two TRBV-D-J-C loci in opposite orientation on two different chromosomes. Our data shed light on the evolution of TRB loci in Salmonids after their whole genome duplication (WGD). We established a coherent nomenclature of salmonid TRB loci based on comprehensive annotation. Our work provides a fundamental basis for monitoring salmonid T cell responses by TRB repertoire sequencing.

【 授权许可】

Unknown   
Copyright © 2023 Boudinot, Novas, Jouneau, Mondot, Lefranc, Grimholt and Magadán