Identification of Circular RNA-Based Immunomodulatory Networks in Colorectal Cancer

Frontiers in Oncology

Oncology

Ahao Wu¹  Zhengrong Li²  Yang Zhang²  Lianghua Luo²  Xufeng Shu²  Qingwen Zeng²  Zongfeng Feng²  Wenzheng Chen²  Yi Cao²  Yi Tu³  Leyan Li⁴ 

[1]Department of General Surgery, First Affiliated Hospital of Nanchang University, Nanchang, China

[2]Laboratory of Digestive Surgery, Nanchang University, Nanchang, China

[3]Department of General Surgery, First Affiliated Hospital of Nanchang University, Nanchang, China

[4]Laboratory of Digestive Surgery, Nanchang University, Nanchang, China

[5]Medical Innovation Center, the First Affiliated Hospital of Nanchang University, Nanchang, China

[6]Department of Pathology, the First Affiliated Hospital of Nanchang University, Nanchang, China

[7]Laboratory of Digestive Surgery, Nanchang University, Nanchang, China

[8]Medical Innovation Center, the First Affiliated Hospital of Nanchang University, Nanchang, China

[9]Queen Mary School, Medical Department of Nanchang University, Nanchang, China

关键词: circRNA;    RPPH1;    ceRNA network;    RBP;    M2 macrophages;    Immunomodulatory;   

DOI  :  10.3389/fonc.2021.779706

 received in 2021-09-19, accepted in 2021-12-30,  发布年份 2022

来源: Frontiers

【 摘 要 】

BackgroundCircular RNAs (circRNAs) have been recently proposed as hub molecules in various diseases, especially in tumours. We found that circRNAs derived from ribonuclease P RNA component H1 (RPPH1) were highly expressed in colorectal cancer (CRC) samples from Gene Expression Omnibus (GEO) datasets.ObjectiveWe sought to identify new circRNAs derived from RPPH1 and investigate their regulation of the competing endogenous RNA (ceRNA) and RNA binding protein (RBP) networks of CRC immune infiltration.MethodsThe circRNA expression profiles miRNA and mRNA data were extracted from the GEO and The Cancer Genome Atlas (TCGA) datasets, respectively. The differentially expressed (DE) RNAs were identified using R software and online server tools, and the circRNA–miRNA–mRNA and circRNA–protein networks were constructed using Cytoscape. The relationship between targeted genes and immune infiltration was identified using the GEPIA2 and TIMER2 online server tools.ResultsA ceRNA network, including eight circRNAs, five miRNAs, and six mRNAs, was revealed. Moreover, a circRNA–protein network, including eight circRNAs and 49 proteins, was established. The targeted genes, ENOX1, NCAM1, SAMD4A, and ZC3H10, are closely related to CRC tumour-infiltrating macrophages.ConclusionsWe analysed the characteristics of circRNA from RPPH1 as competing for endogenous RNA binding miRNA or protein in CRC macrophage infiltration. The results point towards the development of a new diagnostic and therapeutic paradigm for CRC.

【 授权许可】

Unknown   
Copyright © 2022 Feng, Li, Tu, Shu, Zhang, Zeng, Luo, Wu, Chen, Cao and Li