Genetic analysis and mapping of dwarf gene without yield penalty in a γ-ray-induced wheat mutant

Frontiers in Plant Science

Plant Science

Huijun Guo¹  Jiayu Gu¹  Shirong Zhao¹  Yuping Ding¹  Hongchun Xiong¹  Linshu Zhao¹  Yongdun Xie¹  Luxiang Liu¹  Qingguo Wang² 

[1] National Key Facility for Crop Gene Resources and Genetic Improvement, National Center of Space Mutagenesis for Crop Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China;School of Life Sciences, Qingdao Agricultural University, Qingdao, China;National Key Facility for Crop Gene Resources and Genetic Improvement, National Center of Space Mutagenesis for Crop Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China;

关键词: wheat;    plant height;    dwarf mutant;    QTL;    γ-ray;   

DOI  :  10.3389/fpls.2023.1133024

 received in 2022-12-28, accepted in 2023-02-28,  发布年份 2023

来源: Frontiers

【 摘 要 】

Plant height is one of the most important agronomic traits that affects yield in wheat, owing to that the utilization of dwarf or semi-dwarf genes is closely associated with lodging resistance. In this study, we identified a semi-dwarf mutant, jg0030, induced by γ-ray mutagenesis of the wheat variety ‘Jing411’ (wild type). Compared with the ‘Jing411’, plant height of the jg0030 mutant was reduced by 7%-18% in two years’ field experiments, and the plants showed no changes in yield-related traits. Treatment with gibberellic acid (GA) suggested that jg0030 is a GA-sensitive mutant. Analysis of the frequency distribution of plant height in 297 F3 families derived from crossing jg0030 with the ‘Jing411’ indicated that the semi-dwarf phenotype is controlled by a major gene. Using the wheat 660K SNP array-based Bulked Segregant Analysis (BSA) and the exome capture sequencing-BSA assay, the dwarf gene was mapped on the long arm of chromosome 2B. We developed a set of KASP markers and mapped the dwarf gene to a region between marker PH1 and PH7. This region encompassed a genetic distance of 55.21 cM, corresponding to a physical distance of 98.3 Mb. The results of our study provide a new genetic resource and linked markers for wheat improvement in molecular breeding programs.

【 授权许可】

Unknown   
Copyright © 2023 Wang, Xiong, Guo, Zhao, Xie, Gu, Zhao, Ding and Liu