Bacterial and fungal diversities examined through high-throughput sequencing in response to lead contamination of tea garden soil

Frontiers in Microbiology

Microbiology

Hui Ye¹  Gaofei Ge¹  Qingmei Deng²  Ziyan Zhang² 

[1] Biotechnology Centre, Anhui Agricultural University, Hefei, China;School of Resources and Environment, Anhui Agricultural University, Hefei, China;

关键词: multistage contamination;    single instance contamination;    bacterial diversity;    fungal diversity;    high-throughput sequencing;    lead;   

DOI  :  10.3389/fmicb.2023.1121199

 received in 2022-12-11, accepted in 2023-03-07,  发布年份 2023

来源: Frontiers

【 摘 要 】

Several studies have indicated that the heavy-metal content in tea is increasing gradually. Researchers examining the soil of more than 100 tea gardens in China have observed that lead content was higher in some soils. The effect of lead contamination on soil microorganisms in tea gardens was studied to determine the effect of lead on the essential functions of microorganisms in a tea garden soil ecosystem. Previous studies on pot experiments adopted the method of adding a single instance of pollution, which failed to comprehensively simulate the characteristics of the slow accumulation of heavy metals in soil. This study designed with two pollution modes (multistage and single instance) determined the content of soil lead in different forms according to the European Community Bureau of Reference extraction procedure. The community structure, species diversity and functional abundance of soil bacteria and fungi were examined by high-throughput sequencing. We observed that the content of four forms of lead was higher in the multistage contamination mode than in the single instance contamination mode. The effects of lead contamination on bacteria differed significantly (p < 0.05), and the abundance and diversity of bacteria were higher in the multistage contamination mode than in the single instance contamination mode. The community structure of fungi was more affected by lead than was that of bacteria. The content of each lead form was the environmental factor most strongly affecting soil bacteria and fungi. The predicted main function of the bacterial community was amino acid transport and metabolism, and the trophic mode of the fungal community was mainly pathotroph–saprotroph. This study revealed changes in soil microorganisms caused by different forms of lead and contamination methods in tea garden soil and provide a theoretical basis for examining the effects of lead contamination on soil microorganisms.

【 授权许可】

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Copyright © 2023 Zhang, Deng, Ye and Ge.