Homologous genes shared between probiotics and pathogens affect the adhesion of probiotics and exclusion of pathogens in the gut mucus of shrimp

Frontiers in Microbiology

Microbiology

Guomin Wang¹  Weiying Dong¹  Shiying Zheng²  Jiafeng Yu²  Jie Qu²  Jian Liu²  Yujie Sha²  Shicai Xu²  Jing Ren²  Qingyun Yan³  Zhili He³ 

[1] Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, China;Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, China;Shandong Engineering Laboratory of Swine Health Big Data and Intelligent Monitoring, Institute of Biophysics, Dezhou University, Dezhou, China;Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, China;

关键词: Lactiplantibacillus plantarum;    probiotics;    mucus;    adhesion;    competitive exclusion;   

DOI  :  10.3389/fmicb.2023.1195137

 received in 2023-03-28, accepted in 2023-05-29,  发布年份 2023

来源: Frontiers

【 摘 要 】

Clarifying mechanisms underlying the selective adhesion of probiotics and competitive exclusion of pathogens in the intestine is a central theme for shrimp health. Under experimental manipulation of probiotic strain (i.e., Lactiplantibacillus plantarum HC-2) adhesion to the shrimp mucus, this study tested the core hypothesis that homologous genes shared between probiotic and pathogen would affect the adhesion of probiotics and exclusion of pathogens by regulating the membrane proteins of probiotics. Results indicated that the reduction of FtsH protease activity, which significantly correlated with the increase of membrane proteins, could increase the adhesion ability of L. plantarum HC-2 to the mucus. These membrane proteins mainly involved in transport (glycine betaine/carnitine/choline ABC transporter choS, ABC transporter, ATP synthase subunit a atpB, amino acid permease) and regulation of cellular processes (histidine kinase). The genes encoding the membrane proteins were significantly (p < 0.05) up-regulated except those encoding ABC transporters and histidine kinases in L. plantarum HC-2 when co-cultured with Vibrio parahaemolyticus E1, indicating that these genes could help L. plantarum HC-2 to competitively exclude pathogens. Moreover, an arsenal of genes predicted to be involved in carbohydrate metabolism and bacteria-host interactions were identified in L. plantarum HC-2, indicating a clear strain adaption to host’s gastrointestinal tract. This study advances our mechanistic understanding of the selective adhesion of probiotics and competitive exclusion of pathogens in the intestine, and has important implications for screening and applying new probiotics for maintaining gut stability and host health.

【 授权许可】

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Copyright © 2023 Sha, Yan, Liu, Yu, Xu, He, Ren, Qu, Zheng, Wang and Dong.