Antennal transcriptomic analysis of carboxylesterases and glutathione S-transferases associated with odorant degradation in the tea gray geometrid, Ectropis grisescens (Lepidoptera, Geometridae)

Frontiers in Physiology

Physiology

Yanan Zhi¹  Zhou Zhou¹  Xiaocen Zhao¹  Feng Hong¹  Li Zhang¹  Shibao Guo¹  Yijun Chen²  Fangmei Zhang³  Xuguo Zhou⁴  Yunhui Zhang⁵  Xiangrui Li⁵ 

[1] College of Agriculture, Xinyang Agriculture and Forestry University, Xinyang, China;College of Agriculture, Xinyang Agriculture and Forestry University, Xinyang, China;College of Agriculture, Xinjiang Agricultural University, Urumqi, China;College of Agriculture, Xinyang Agriculture and Forestry University, Xinyang, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China;Department of Entomology, University of Kentucky, Lexington, KY, United states;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China;

关键词: Ectropis grisescens;    antennal transcriptome;    odorant-degrading enzyme;    carboxylesterases;    glutathione S-transferases;   

DOI  :  10.3389/fphys.2023.1183610

 received in 2023-03-10, accepted in 2023-03-21,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: Carboxylesterases (CXEs) and glutathione S-transferases (GSTs) can terminate olfactory signals during chemosensation by rapid degradation of odorants in the vicinity of receptors. The tea grey geometrid, Ectropis grisescens (Lepidoptera, Geometridae), one of the most devastating insect herbivores of tea plants in China, relies heavily on plant volatiles to locate the host plants as well as the oviposition sites. However, CXEs and GSTs involved in signal termination and odorant clearance in E. grisescens remains unknown.Methods: In this study, identification and spatial expression profiles of CXEs and GSTs in this major tea pest were investigated by transcriptomics and qRT-PCR, respectively.Results: As a result, we identified 28 CXEs and 16 GSTs from female and male antennal transcriptomes. Phylogenetic analyses clustered these candidates into several clades, among which antennal CXEs, mitochondrial and cytosolic CXEs, and delta group GSTs contained genes commonly associated with odorants degradation. Spatial expression profiles showed that most CXEs (26) were expressed in antennae. In comparison, putative GSTs exhibited a diverse expression pattern across different tissues, with one GST expressed specifically in the male antennae.Disscussion: These combined results suggest that 12 CXEs (EgriCXE1, 2, 4, 6, 8, 18, 20-22, 24, 26, and 29) and 5 GSTs (EgriGST1 and EgriGST delta group) provide a major source of candidate genes for odorants degradation in E. grisescens.

【 授权许可】

Unknown   
Copyright © 2023 Zhang, Chen, Zhao, Guo, Hong, Zhi, Zhang, Zhou, Zhang, Zhou and Li.