Rhizosphere-dwelling halophilic archaea: a potential candidate for alleviating salinity-associated stress in agriculture

Frontiers in Microbiology

Microbiology

Monendra Grover¹  Mayur G. Naitam²  B. Ramakrishnan²  Rajeev Kaushik² 

[1] Center for Agricultural Bioinformatics, ICAR-Indian Agricultural Statistical Research Institute, New Delhi, India;Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi, India;

关键词: halophilic archaea;    wheat;    salinity alleviation;    abiotic stress;    plant growth promotion;   

DOI  :  10.3389/fmicb.2023.1212349

 received in 2023-04-26, accepted in 2023-06-30,  发布年份 2023

来源: Frontiers

【 摘 要 】

Salinity is a serious environmental factor that impedes crop growth and drastically reduces yield. This study aimed to investigate the potential of halophilic archaea isolated from the Rann of Kutch to alleviate the negative impact of salinity on crop growth and yield. The halophilic archaea, which demonstrated high tolerance to salinity levels up to 4.5 M, were evaluated for their ability to promote plant growth in both salt-tolerant and salt-susceptible wheat cultivars. Our assessment focused on their capacity to solubilize essential nutrients, including phosphorus (14-61 mg L−1), potassium (37-78 mg L−1), and zinc (8-17 mg L−1), as well as their production of the phytohormone IAA (17.30 to 49.3 μg ml−1). To conduct the experiments, five wheat cultivars (two salt-tolerant and three salt-susceptible) were grown in triplicates using soft MS agar tubes (50 ml) and pots containing 10 kg of soil with an electrical conductivity (EC) of 8 dSm−1. Data were collected at specific time points: 21 days after sowing (DAS) for the MS agar experiment, 45 DAS for the pot experiment, and at the time of harvest. In the presence of haloarchaea, the inoculated treatments exhibited significant increases in total protein (46%), sugar (27%), and chlorophyll (31%) levels compared to the un-inoculated control. Furthermore, the inoculation led to an elevated accumulation of osmolyte proline (31.51%) and total carbohydrates (27.85%) while substantially reducing the activity of antioxidant enzymes such as SOD, catalase, and peroxidase by 57–76%, respectively. Notably, the inoculated treatments also showed improved plant vegetative growth parameters compared to the un-inoculated treatments. Interestingly, the positive effects of the halophilic archaea were more pronounced in the susceptible wheat cultivars than in the tolerant cultivars. These findings highlight the growth-promoting abilities of the halophilic archaeon Halolamina pelagica CDK2 and its potential to mitigate the detrimental effects of salinity. Consequently, further evaluation of this halophilic archaeon under field conditions is warranted to explore its potential use in the development of microbial inoculants.

【 授权许可】

Unknown   
Copyright © 2023 Naitam, Ramakrishnan, Grover and Kaushik.