Carbon dots labeled Lactiplantibacillus plantarum: a fluorescent multifunctional biocarrier for anticancer drug delivery

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Ahmed A. Hussein¹  Hoda E. Mahmoud¹  Noor A. Abdullah¹  Nefertiti A. El-Nikhely¹  Labiba K. El-Khordagui² 

[1]Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt

[2]Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt

关键词: bioimaging;    probiotics;    prodigiosin;    L. plantarum;    Caco-2 cells;    MTT assay;    apoptosis;    molecular docking;   

DOI  :  10.3389/fbioe.2023.1166094

 received in 2023-02-14, accepted in 2023-05-15,  发布年份 2023

来源: Frontiers

【 摘 要 】

A carbon dots (CDs)-biolabeled heat-inactivated Lactiplantibacillus plantarum (HILP) hybrid was investigated as a multifunctional probiotic drug carrier with bioimaging properties using prodigiosin (PG) as anticancer agent. HILP, CDs and PG were prepared and characterized using standard methods. CDs-labeled HILP (CDs/HILP) and PG loaded CDs/HILP were characterized by transmission electron microscopy (TEM), laser scanning confocal microscopy (LSCM) and for entrapment efficiency (EE%) of CDs and PG, respectively. PG-CDs/HILP was examined for stability and PG release. the anticancer activity of PG-CDs/HILP was assessed using different methods. CDs imparted green fluorescence to HILP cells and induced their aggregation. HILP internalized CDs via membrane proteins, forming a biostructure with retained fluorescence in PBS for 3 months at 4°C. Loading PG into CDs/HILP generated a stable green/red bicolor fluorescent combination permitting tracking of both drug carrier and cargo. Cytotoxicity assay using Caco-2 and A549 cells revealed enhanced PG activity by CDs/HILP. LCSM imaging of PG-CDs/HILP-treated Caco-2 cells demonstrated improved cytoplasmic and nuclear distribution of PG and nuclear delivery of CDs. CDs/HILP promoted PG-induced late apoptosis of Caco-2 cells and reduced their migratory ability as affirmed by flow cytometry and scratch assay, respectively. Molecular docking indicated PG interaction with mitogenic molecules involved in cell proliferation and growth regulation. Thus, CDs/HILP offers great promise as an innovative multifunctional nanobiotechnological biocarrier for anticancer drug delivery. This hybrid delivery vehicle merges the physiological activity, cytocompatibility, biotargetability and sustainability of probiotics and the bioimaging and therapeutic potential of CDs.

【 授权许可】

Unknown   
Copyright © 2023 Abdullah, Mahmoud, El-Nikhely, Hussein and El-Khordagui.