A Novel Potent Carrier for Unconventional Protein Export in Ustilago maydis

Frontiers in Cell and Developmental Biology

Cell and Developmental Biology

Michael Feldbrügge¹  Kira Müntjes¹  Kai P. Hussnaetter¹  Magnus Philipp¹  Michèle Reindl¹  Kerstin Schipper² 

[1] Institute for Microbiology, Heinrich Heine University Düsseldorf, Düsseldorf, Germany;null;

关键词: luciferase;    anti-Sars-CoV2 nanobody;    unconventional secretion;    Ustilago maydis;    sybody;   

DOI  :  10.3389/fcell.2021.816335

 received in 2021-11-16, accepted in 2021-12-17,  发布年份 2022

来源: Frontiers

【 摘 要 】

Recombinant proteins are ubiquitously applied in fields like research, pharma, diagnostics or the chemical industry. To provide the full range of useful proteins, novel expression hosts need to be established for proteins that are not sufficiently produced by the standard platform organisms. Unconventional secretion in the fungal model Ustilago maydis is an attractive novel option for export of heterologous proteins without N-glycosylation using chitinase Cts1 as a carrier. Recently, a novel factor essential for unconventional Cts1 secretion termed Jps1 was identified. Here, we show that Jps1 is unconventionally secreted using a fusion to bacterial β-glucuronidase as an established reporter. Interestingly, the experiment also demonstrates that the protein functions as an alternative carrier for heterologous proteins, showing about 2-fold higher reporter activity than the Cts1 fusion in the supernatant. In addition, Jps1-mediated secretion even allowed for efficient export of functional firefly luciferase as a novel secretion target which could not be achieved with Cts1. As an application for a relevant pharmaceutical target, export of functional bi-specific synthetic nanobodies directed against the SARS-CoV2 spike protein was demonstrated. The establishment of an alternative efficient carrier thus constitutes an excellent expansion of the existing secretion platform.

【 授权许可】

Unknown   
Copyright © 2022 Philipp, Hussnaetter, Reindl, Müntjes, Feldbrügge and Schipper.