Bacteria assisted green synthesis of copper oxide nanoparticles and their potential applications as antimicrobial agents and plant growth stimulants

Frontiers in Chemistry

Chemistry

Santosh Ranjan Mohanty¹  Abhijeet Singh²  Sudhir K. Upadhyay³  Wajid Zaman⁴  Deepak Rajpurohit⁵  Deepak Singh⁵  Devendra Jain⁵  Gajanand Jat⁶  Mohammad Khalid Al-Sadoon⁷  Himmat Singh Kushwaha⁸ 

[1] All India Network Project on Soil Biodiversity-Biofertilizers, ICAR-Indian Institute of Soil Science, Bhopal, India;Department of Biosciences, Manipal University Jaipur, Jaipur, India;Department of Environmental Science, V. B. S. Purvanchal University, Jaunpur, India;Department of Life Sciences, Yeungnam University, Gyeongsan, Republic of Korea;Department of Molecular Biology and Biotechnology, Maharana Pratap University of Agriculture and Technology, Udaipur, India;Department of Soil Science and Agricultural Chemistry, Maharana Pratap University of Agriculture and Technology, Udaipur, India;Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia;Material Research Centre, Malviya National Institute of Technology, Jaipur, India;

关键词: novel bacterial isolate;    16s-rDNA sequencing;    CuO-NPs-green synthesis;    confirmatory tests;    antimicrobial and plant growth-promoting activity;   

DOI  :  10.3389/fchem.2023.1154128

 received in 2023-01-30, accepted in 2023-03-23,  发布年份 2023

来源: Frontiers

【 摘 要 】

Copper oxide nanoparticles (CuO-NPs) have piqued the interest of agricultural researchers due to their potential application as fungicides, insecticides, and fertilizers. The Serratia sp. ZTB29 strain, which has the NCBI accession number MK773873, was a novel isolate used in this investigation that produced CuO-NPs. This strain can survive concentrations of copper as high as 22.5 mM and can also remove copper by synthesizing pure CuO-NPs. UV-VIS spectroscopy, DLS, Zeta potential, FTIR, TEM, and XRD techniques were used to investigate the pure form of CuO-NPs. The synthesized CuO-NPs were crystalline in nature (average size of 22 nm) with a monoclinic phase according to the XRD pattern. CuO-NPs were found to be polydisperse, spherical, and agglomeration-free. According to TEM and DLS inspection, they ranged in size from 20 to 40 nm, with a typical particle size of 28 nm. CuO-NPs were extremely stable, as demonstrated by their zeta potential of −15.4 mV. The ester (C=O), carboxyl (C=O), amine (NH), thiol (S-H), hydroxyl (OH), alkyne (C-H), and aromatic amine (C-N) groups from bacterial secretion were primarily responsible for reduction and stabilization of CuO-NPs revealed in an FTIR analysis. CuO-NPs at concentrations of 50 μg mL−1 and 200 μg mL−1 displayed antibacterial and antifungal activity against the plant pathogenic bacteria Xanthomonas sp. and pathogenic fungus Alternaria sp., respectively. The results of this investigation support the claims that CuO-NPs can be used as an efficient antimicrobial agent and nano-fertilizer, since, compared to the control and higher concentrations of CuO-NPs (100 mg L−1) considerably improved the growth characteristics of maize plants.

【 授权许可】

Unknown   
Copyright © 2023 Singh, Jain, Rajpurohit, Jat, Kushwaha, Singh, Mohanty, Al-Sadoon, Zaman and Upadhyay.