Glabridin inhibited the spread of polymyxin-resistant Enterobacterium carrying ICEMmoMP63

Frontiers in Microbiology

Microbiology

Jiafang Fu¹  Yayu Liu¹  Guangxiang Cao¹  Gongli Zong¹  Fengtian Wang²  Chuanqing Zhong³  Zhen Wang³ 

[1] First Affiliated Hospital of Shandong First Medical University, Biomedical Sciences College & Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, China;National Health Commission (NHC) Key Laboratory of Biotechnology Drugs. Shandong Academy of Medical Sciences, Jinan, China;Jinan Municipal Minzu Hospital, Jinan, China;School of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan, China;

关键词: Morganella morganii;    polymyxin resistance;    integrative and conjugative element;    MFS transporter;    glabridin;   

DOI  :  10.3389/fmicb.2023.1188900

 received in 2023-03-18, accepted in 2023-05-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionThe role of integrative and conjugative elements (ICEs) in antibiotic resistance in Morganella morganii is unknown. This study aimed to determine whether an ICE identified in the M. morganii genome contributed to the polymyxin resistance.MethodsWhole-genome sequencing was performed followed by bioinformatics analyses to identify ICEs and antibiotic resistance genes. Conjugation assays were performed to analyze the transferability of a discovered ICE. A drug transporter encoded on the ICE was heterogeneously expressed in Escherichia coli, minimum inhibitory concentrations of antibiotics were determined, and a traditional Chinese medicine library was screened for potential efflux pump inhibitors.ResultsAn antibiotic resistance-conferring ICE, named ICEMmoMP63, was identified. ICEMmoMP63 was verified to be horizontally transferred among Enterobacteriaceae bacteria. G3577_03020 in ICEMmoMP63 was found to mediate multiple antibiotic resistances, especially polymyxin resistance. However, natural compound glabridin was demonstrated to inhibit polymyxin resistance.DiscussionOur findings support the need for monitoring dissemination of ICEMmoMP63 in Enterobacteriaceae bacteria. Combined glabridin and polymyxin may have therapeutic potential for treating infections from multi-drug resistant bacteria carrying ICEMmoMP63.

【 授权许可】

Unknown   
Copyright © 2023 Fu, Liu, Wang, Zong, Wang, Zhong and Cao.