Identification of microRNAs implicated in modulating resveratrol-induced apoptosis in porcine granulosa cells

Frontiers in Cell and Developmental Biology

Cell and Developmental Biology

Jinglin Wang¹  Mengyao Qiu¹  Zheng Han¹  Xiaodong Zhang¹  Yueyun Ding¹  Huibin Zhang¹  Zongjun Yin¹  Yangguang Liu¹  Xianrui Zheng¹  Fan Xie¹ 

[1] College of Animal Science and Technology, Anhui Agricultural University, Hefei, China;Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, Hefei, China;

关键词: miRNA;    resveratrol;    apoptosis;    ovary;    pig;   

DOI  :  10.3389/fcell.2023.1169745

 received in 2023-02-20, accepted in 2023-04-27,  发布年份 2023

来源: Frontiers

【 摘 要 】

MicroRNAs (miRNAs) are small, noncoding RNAs that play a crucial role in the complex and dynamic network that regulates the apoptosis of porcine ovarian granulosa cells (POGCs). Resveratrol (RSV) is a nonflavonoid polyphenol compound that is involved in follicular development and ovulation. In previous study, we established a model of RSV treatment of POGCs, confirming the regulatory effect of RSV in POGCs. To investigate the miRNA-level effects of RSV on POGCs to reveal differentially expressed miRNAs, a control group (n = 3, 0 μM RSV group), a low RSV group (n = 3, 50 μM RSV group), and a high RSV group (n = 3, 100 μM RSV group) were created for small RNA-seq. In total, 113 differentially expressed miRNAs (DE-miRNAs) were identified, and a RT-qPCR analysis showed a correlation with the sequencing data. Functional annotation analysis revealed that DE-miRNAs in the LOW vs. CON group may be involved in cell development, proliferation, and apoptosis. In the HIGH vs. CON group, RSV functions were associated with metabolic processes and responses to stimuli, while the pathways were related to PI3K24, Akt, Wnt, and apoptosis. In addition, we constructed miRNA-mRNA networks related to Apoptosis and Metabolism. Then, ssc-miR-34a and ssc-miR-143-5p were selected as key miRNAs. In conclusion, this study provided an improved understanding of effects of RSV on POGCs apoptosis through the miRNA modulations. The results suggest that RSV may promote POGCs apoptosis by stimulating the miRNA expressions and provided a better understanding of the role of miRNAs combined with RSV in ovarian granulosa cell development in pigs.

【 授权许可】

Unknown   
Copyright © 2023 Zhang, Wang, Xie, Liu, Qiu, Han, Ding, Zheng, Yin and Zhang.