Culturable bacterial endophytes of Aconitum carmichaelii Debx. were diverse in phylogeny, plant growth promotion, and antifungal potential

Frontiers in Microbiology

Microbiology

Wei Dai¹  Kunhao Ye¹  Lan Zou²  Jing Huang²  Muyi Li²  Qian Wang²  Siyu Wang² 

[1] Institute of Traditional Chinese Medicinal Materials, Miangyang Academy of Agricultural Science, Mianyang, China;School of Life Science and Engineering, Southwest University of Science and Technology, Miangyang, China;

关键词: biological control;    endophytic bacteria;    genetic diversity;    plant growth promotion;    Sclerotium rolfsii;   

DOI  :  10.3389/fmicb.2023.1192932

 received in 2023-03-24, accepted in 2023-04-21,  发布年份 2023

来源: Frontiers

【 摘 要 】

Medicinal plants harbor tremendously diverse bacterial endophytes that maintain plant growth and health. In the present study, a total of 124 culturable bacterial endophytes were isolated from healthy Aconitum carmichaelii Debx. plants. These strains were clustered into 10 genera based on full-length 16S rDNA sequences, among which Bacillus and Pseudomonas were the dominant genera. In addition, A. carmichaelii may capture 10 potential new bacterial species based on multi-locus sequence analysis of three housekeeping genes (gyrA, rpoB, and atpD). The majority of these bacterial endophytes exhibited plant growth-promoting ability through diverse actions including the production of either indole acetic acid and siderophore or hydrolytic enzymes (glucanase, cellulose, and protease) and solubilization of phosphate or potassium. A total of 20 strains inhibited hyphal growth of fungal pathogens Sclerotium rolfsii and Fusarium oxysporum in vitro on root slices of A. carmichaelii by the dual-culture method, among which Pseudomonas sp. SWUSTb-19 showed the best antagonistic activity. Field experiment confirmed that Pseudomonas sp. SWUSTb-19 significantly reduced the occurrence of southern blight and promoted plant biomass compared with non-inoculation treatment. The possible mode of actions for Pseudomonas sp. SWUSTb-19 to antagonize against S. rolfsii involved the production of glucanase, siderophore, lipopeptides, and antimicrobial volatile compounds. Altogether, this study revealed that A. carmichaelii harbored diverse plant growth-promoting bacterial endophytes, and Pseudomonas sp. SWUSTb-19 could be served as a potential biocontrol agent against southern blight.

【 授权许可】

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Copyright © 2023 Zou, Wang, Li, Wang, Ye, Dai and Huang.