Prognostic and tumor microenvironmental feature of clear cell renal cell carcinoma revealed by m6A and lactylation modification-related genes

Frontiers in Immunology

Immunology

Jiahao Liu¹  Bin Fu¹  Songhui Xu¹  Qianxi Dong¹  Fuchun Zheng¹  Xiaoyu Wang¹  Xiaoqiang Liu¹  Jing Xiong¹  Lin Yang¹  Sheng Li¹ 

[1] Department of Urology, First Affiliated Hospital of Nanchang University, Nanchang, China;

关键词: lactylation;    M6A;    clear cell renal cell carcinoma;    immunotherapy;    tumor microenvironment;   

DOI  :  10.3389/fimmu.2023.1225023

 received in 2023-05-18, accepted in 2023-07-26,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundBoth lactylation and m6A modification have important implications for the development of clear cell renal cell carcinoma (ccRCC), and we aimed to use crosstalk genes of both to reveal the prognostic and immunological features of ccRCC.MethodsOur first step was to look for lactylation-related genes that differed between normal and tumor tissues, and then by correlation analysis, we found the genes associated with M6A. Following that, ccRCC subtypes will be identified and risk models will be constructed to compare the prognosis and tumor microenvironment among different subgroups. A nomogram was constructed to predict the prognosis of ccRCC, and in vitro, experiments were conducted to validate the expression and function of key genes.ResultsWe screened 100 crosstalk genes and identified 2 ccRCC subtypes. A total of 11 prognostic genes were screened for building a risk model. we observed higher immune scores, elevated tumor mutational burden, and microsatellite instability scores in the high-risk group. Therefore, individuals classified as high-risk would derive greater benefits from immunotherapy. The nomogram’s ability to predict overall survival with a 1-year AUC of 0.863 demonstrates its significant practical utility. In addition, HIBCH was identified as a potential therapeutic target and its expression and function were verified by in vitro experiments.ConclusionIn addition to developing a precise prognostic nomogram for patients with ccRCC, our study also discovered the potential of HIBCH as a biomarker for the disease.

【 授权许可】

Unknown   
Copyright © 2023 Yang, Wang, Liu, Liu, Li, Zheng, Dong, Xu, Xiong and Fu