A possible genetic association between obesity and colon cancer in females

Frontiers in Endocrinology

Endocrinology

Yi Gong¹  Ruo Shu²  Yan Tian²  Xin-feng Zhang²  Hua-you Luo²  Xiao-li Zhang²  Yuan Fang³  Meng-li Li⁴ 

[1] Basic Medical Sciences, Kunming Medical University, Kunming, China;Department of Gastrointestinal and Hernia Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming, China;Department of Hepatobiliary Transplantation, The First Affiliated Hospital of Kunming Medical University, Kunming, China;Department of Respiratory Medicine, Affiliated Hospital of Yunnan University, Kunming, China;

关键词: obesity;    colon cancer;    women;    GEO;    genes;   

DOI  :  10.3389/fendo.2023.1189570

 received in 2023-03-19, accepted in 2023-08-07,  发布年份 2023

来源: Frontiers

【 摘 要 】

ObjectThere is mounting clinical evidence that an increase in obesity is linked to an increase in cancer incidence and mortality. Although studies have shown a link between obesity and colon cancer, the particular mechanism of the interaction between obesity and colon cancer in females remains unknown. The goal of this work is to use bioinformatics to elucidate the genetic link between obesity and colon cancer in females and to investigate probable molecular mechanisms.MethodsGSE44076 and GSE199063 microarray datasets were obtained from the Gene Expression Omnibus (GEO) database. In the two microarray datasets and healthy controls, the online tool GEO2R was utilized to investigate the differential genes between obesity and colon cancer. The differential genes (DEGs) identified in the two investigations were combined. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment studies were performed on the DEGs. The STRING database and Cytoscape software were then used to build protein-protein interaction (PPI) networks to discover hub genes. NetworkAnalyst was also used to build networks of target microRNAs (miRNAs) and hub genes, as well as networks of transcriptions.ResultsBetween the two datasets, 146 DEGs were shared. The DEGs are primarily enriched in inflammatory and immune-related pathways, according to GO analysis and KEGG. 14 hub genes were identified via PPI building using the Cytoscape software’s MCODE and CytoNCA plug-ins: TYROBP, CD44, BGN, FCGR3A, CD53, CXCR4, FN1, SPP1, IGF1, CCND1, MMP9, IL2RG, IL6 and CTGF. Key transcription factors for these hub genes include WRNIP1, ATF1, CBFB, and NR2F6. Key miRNAs for these hub genes include hsa-mir-1-3p, hsa-mir-26b-5p, hsa-mir-164a-5p and hsa-mir-9-5p.ConclusionOur research provides evidence that changed genes are shared by female patients with colon cancer and obesity. Through pathways connected to inflammation and the immune system, these genes play significant roles in the emergence of both diseases. We created a network between hub genes and miRNAs that target transcription factors, which may offer suggestions for future research in this area.

【 授权许可】

Unknown   
Copyright © 2023 Zhang, Zhang, Fang, Li, Shu, Gong, Luo and Tian