Identification of two novel papillomaviruses in belugas

Frontiers in Microbiology

Microbiology

Xinran Song¹  Meifang Xiao²  Zihan Li³  Xiaoyuan Hu³  Chuanning Tang³  Youyou Li³  Yun Zhang³  Gang Lu³  Ruoyan Peng³  Gaoyu Wang³  Feifei Yin⁴  Jiang Du⁵  Shijie Bai⁶  Peijun Zhang⁶  Haoxiang Su⁷  Xin Li⁸ 

[1] Dalian Sun Asia Tourism Holding Co., Ltd., Dalian, China;Department of Clinical Laboratory, Center for Laboratory Medicine, Hainan Women and Children’s Medical Center, Haikou, China;Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China;Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China;Department of Clinical Laboratory, Center for Laboratory Medicine, Hainan Women and Children’s Medical Center, Haikou, China;Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China;National Health Commission, Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China;Marine Mammal and Marine Bioacoustics Laboratory, Laboratory of Marine Viruses and Molecular Biology, Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya, China;National Health Commission, Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China;Qingdao Polar Haichang Ocean Park, Qingdao, China;

关键词: diversity;    novel PVs;    evolution;    beluga whale;    next-generation sequencing;   

DOI  :  10.3389/fmicb.2023.1165839

 received in 2023-02-14, accepted in 2023-07-03,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionPapillomaviruses (PVs) can cause hyperplasia in the skin and mucous membranes of humans, mammals, and non-mammalian animals, and are a significant risk factor for cervical and genital cancers.MethodsUsing next-generation sequencing (NGS), we identified two novel strains of papillomavirus, PV-HMU-1 and PV-HMU-2, in swabs taken from belugas (Delphinapterus leucas) at Polar Ocean Parks in Qingdao and Dalian.ResultsWe amplified the complete genomes of both strains and screened ten belugas and one false killer whale (Pseudorca crassidens) for the late gene (L1) to determine the infection rate. In Qingdao, 50% of the two sampled belugas were infected with PV-HMU-1, while the false killer whale was negative. In Dalian, 71% of the eight sampled belugas were infected with PV-HMU-2. In their L1 genes, PV-HMU-1 and PV-HMU-2 showed 64.99 and 68.12% amino acid identity, respectively, with other members of Papillomaviridae. Phylogenetic analysis of combinatorial amino acid sequences revealed that PV-HMU-1 and PV-HMU-2 clustered with other known dolphin PVs but formed distinct branches. PVs carried by belugas were proposed as novel species under Firstpapillomavirinae.ConclusionThe discovery of these two novel PVs enhances our understanding of the genetic diversity of papillomaviruses and their impact on the beluga population.

【 授权许可】

Unknown   
Copyright © 2023 Li, Xiao, Zhang, Li, Bai, Su, Peng, Wang, Hu, Song, Li, Tang, Lu, Yin, Zhang and Du.