Bacterial community responses of the hydrothermal vent crab Xenograpsus testudinatus fed on microplastics

Frontiers in Ecology and Evolution

Ecology and Evolution

Yun-Cheng Lee¹  Yu-Ling Chen¹  Priyanka Muthu¹  Gowri Krishna Girija¹  Ying-Ning Ho²  Jiang-Shiou Hwang³ 

[1] Institute of Marine Biology, National Taiwan Ocean University, Keelung, Taiwan;Institute of Marine Biology, National Taiwan Ocean University, Keelung, Taiwan;Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan;Institute of Marine Biology, National Taiwan Ocean University, Keelung, Taiwan;Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan;Center of Excellence for Ocean Engineering, National Taiwan Ocean University, Keelung, Taiwan;

关键词: polyethylene;    Oxford Nanopore Technologies;    chemoheterotrophy;    functional group;    Xenograpsus testudinatus;   

DOI  :  10.3389/fevo.2023.1203647

 received in 2023-04-11, accepted in 2023-08-03,  发布年份 2023

来源: Frontiers

【 摘 要 】

Microplastics (MPs) provide persistent contaminants in freshwaters and the oceans from anthropogenic sources worldwide. MP contamination in ecosystems has emerged as a global environmental issue. While increasing research focused on the ecological consequences of plastic pollution, health-related implications of plastic pollution have been somewhat overlooked. In this study, we evaluated the effects of polyethylene MP contamination on microbial, physical, and biochemical characteristics of the hydrothermal vent crab Xenograpsus testudinatus over a 7-day food exposure. Different concentrations (0%, 0.3%, 0.6%, and 1.0%) of polyethylene MPs were used for feed intake experiments. Oxford Nanopore Technologies’ full-length sequencing of the 16S rDNA gene was used to explore the changes of the microbial composition in vent crab tissues. At the phylum level, the content of Firmicutes significantly decreased in the digestive gland tissue. Furthermore, the predicted functions of genes in the microbial community were significantly influenced by MPs. In contrast, there were eight functions in gill and 11 functions in digestive gland tissues identified at low and high intake levels. The dominant functions of methylotrophy, dark thiosulfate oxidation, dark oxidation of sulfur compounds, aromatic hydrocarbon degradation, and aromatic compound degradation were significantly increased at high intake levels in the digestive gland. These findings indicate that MP ingestion causes not only a short-term decrease in energy intake for crustaceans but also a change in microbial communities and their functions. This study provided a first account on the toxicity of MPs in a hydrothermal vent crab to aid in the assessment of health risks provided by polyethylene MP to marine invertebrates.

【 授权许可】

Unknown   
Copyright © 2023 Muthu, Lee, Girija, Chen, Ho and Hwang