Dihydromyricetin functions as a tumor suppressor in hepatoblastoma by regulating SOD1/ROS pathway

Frontiers in Oncology

Oncology

Jinfa Tou¹  Tong Guo¹  Tian Xia²  Xitong Wang²  Gensheng Zhang²  Runzhi Zhu² 

[1] Department of Neonatal Surgery, The Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, China;National Clinical Research Center for Child Health, The Children’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China;

关键词: apoptosis;    hepatoblastoma;    SOD1;    ROS;    anticancer activity;   

DOI  :  10.3389/fonc.2023.1160548

 received in 2023-02-07, accepted in 2023-04-25,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundHepatoblastoma has an unsatisfactory prognosis, and traditional chemotherapy has strong side effects. Dihydromyricetin is a flavonoid extracted from a woody vine of the genus Serpentine in the family Vitaceae, with effects such as preventing alcoholic liver and reducing the incidence of liver cancer. However, the effect of DHM on hepatoblastoma and its specific pathway are still unclear. PurposeThe purpose of this study was to investigate the effects of DHM on children's hepatoblastoma and its related mechanisms.MethodsCCK-8 assays were used to measure proliferation. Apoptosis and reactive oxygen species (ROS) were analyzed by flow cytometry. Apoptotic cells were observed using Hoechst 33342 staining and fluorescence microscopy. Protein expression levels in HuH-6 and HepG2 cells were determined by western blotting.ResultsWe found that DHM was able to inhibit the growth and increase cellular mortality in HuH-6 and HepG2 cells. Furthermore, DHM decreased the intracellular ROS level and increased the expression of SOD1. ROS scavenger NAC promoted apoptosis, while the use of SOD1 inhibitor LCS-1 weakened the ROS scavenging effect of DHM , and to some extent reduced the killing effect of DHM on hepatoblastoma cells. ConclusionThese results suggest that regulating SOD1/ROS pathway to induce apoptosis is one of the potential mechanisms of DHM as a tumor suppressor in hepatoblastoma. Therefore, DHM may be a novel candidate for inhibiting hepatoblastoma growth and deserves further study.

【 授权许可】

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Copyright © 2023 Guo, Wang, Zhang, Xia, Zhu and Tou