Multiparametric flow cytometry to characterize vaccine-induced polyfunctional T cell responses and T cell/NK cell exhaustion and memory phenotypes in mouse immuno-oncology models

Frontiers in Immunology

Immunology

David P. Sester¹  Rituparna Bhatt²  Jack Wood²  Simone A. Minnie³  Davide Moi⁴  Bijun Zeng⁴  Roberta Mazzieri⁵  Riccardo Dolcetti⁶ 

[1] TRI Flow Cytometry Suite, Translational Research Institute, Woolloongabba, QLD, Australia;The University of Queensland Frazer Institute, Woolloongabba, QLD, Australia;The University of Queensland Frazer Institute, Woolloongabba, QLD, Australia;Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, United States;The University of Queensland Frazer Institute, Woolloongabba, QLD, Australia;Peter MacCallum Cancer Centre, Melbourne, VIC, Australia;The University of Queensland Frazer Institute, Woolloongabba, QLD, Australia;Peter MacCallum Cancer Centre, Melbourne, VIC, Australia;Sir Peter MacCallum Department of Oncology, The University of Melbourne, Melbourne, VIC, Australia;The University of Queensland Frazer Institute, Woolloongabba, QLD, Australia;Peter MacCallum Cancer Centre, Melbourne, VIC, Australia;Sir Peter MacCallum Department of Oncology, The University of Melbourne, Melbourne, VIC, Australia;Department of Microbiology and Immunology, The University of Melbourne, Melbourne, VIC, Australia;

关键词: immunomonitoring;    polyfunctional T cell assay;    multiparametric flow cytometry;    cancer vaccines;    mouse cancer models;    cancer immunotherapy;    immune checkpoints;   

DOI  :  10.3389/fimmu.2023.1127896

 received in 2022-12-20, accepted in 2023-03-23,  发布年份 2023

来源: Frontiers

【 摘 要 】

Suitable methods to assess in vivo immunogenicity and therapeutic efficacy of cancer vaccines in preclinical cancer models are critical to overcome current limitations of cancer vaccines and enhance the clinical applicability of this promising immunotherapeutic strategy. In particular, availability of methods allowing the characterization of T cell responses to endogenous tumor antigens is required to assess vaccine potency and improve the antigen formulation. Moreover, multiparametric assays to deeply characterize tumor-induced and therapy-induced immune modulation are relevant to design mechanism-based combination immunotherapies. Here we describe a versatile multiparametric flow cytometry method to assess the polyfunctionality of tumor antigen-specific CD4+ and CD8+ T cell responses based on their production of multiple cytokines after short-term ex vivo restimulation with relevant tumor epitopes of the most common mouse strains. We also report the development and application of two 21-color flow cytometry panels allowing a comprehensive characterization of T cell and natural killer cell exhaustion and memory phenotypes in mice with a particular focus on preclinical cancer models.

【 授权许可】

Unknown   
Copyright © 2023 Moi, Zeng, Minnie, Bhatt, Wood, Sester, Mazzieri and Dolcetti