Corylin accelerated wound healing through SIRT1 and PI3K/AKT signaling: a candidate remedy for chronic non-healing wounds

Frontiers in Pharmacology

Pharmacology

Yuhang Li¹  Hui Yuan¹  Lihua Gao²  Yanghui Xiu²  Sennan Xu²  Ying Liu²  Yu Su²  Yan Qiu²  Zhen Liu² 

[1] CAS Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Provincial Key Laboratory of Nanomaterials, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Xiamen, China;Xiamen Institute of Rare-Earth Materials, Haixi Institutes, Chinese Academy of Sciences, Fujian, China;Eye Institute and Affiliated Xiamen Eye Center of Xiamen University, School of Medicine, Xiamen University, Xiamen, China;Fujian Provincial Key Laboratory of Corneal and Ocular Surface Diseases, Xiamen, Fujian, China;

关键词: corylin;    phosphatidylinositol-3-kinase (PI3K);    protein kinase B (AKT);    sirtuin 1 (SIRT1);    wound healing;   

DOI  :  10.3389/fphar.2023.1153810

 received in 2023-01-30, accepted in 2023-05-04,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: Chronic non-healing wound is a considerable clinical challenge and research into the discovery of novel pro-healing agents is underway as existing therapeutic approaches cannot sufficiently meet current needs.Method: We studied the effects of corylin in cell line fibroblasts and macrophages by Western blots, PCR, Flow cytometry assay, Immunofluorescence.Results: We showed that corylin, a main flavonoid extracted from Psoralea corylifolia L, reduced inflammatory responses, promoted collagen deposition, and accelerated the healing of full-thickness skin wounds in mice. Exploration of the underlying mechanisms showed that corylin activated the PI3K/AKT signaling, leading to fibroblasts’ migration, proliferation, and scratch healing. Corylin also activated sirtuin 1 (SIRT1) signaling, enhanced the deacetylation and cytoplasmic translocation of NF-κB p65, and therefore reduced lipopolysaccharide (LPS)-induced inflammatory responses in macrophages. Furthermore, inhibition of PI3K/AKT and sirtuin 1 pathway with LY294002 and EX527 prevent the therapeutic potency of corylin against chronic wounds.Conclusion: In summary, our results suggested that corylin may be a candidate for the development of novel pro-healing agents.

【 授权许可】

Unknown   
Copyright © 2023 Xiu, Su, Gao, Yuan, Xu, Liu, Qiu, Liu and Li.