Degradation of crude oil in a co-culture system of Bacillus subtilis and Pseudomonas aeruginosa

Frontiers in Microbiology

Microbiology

Lina Yi¹  Jianlong Xiu¹  Li Yu¹  Lixin Huang¹  Yuandong Ma¹  Bo Wu² 

[1] PetroChina Research Institute of Petroleum Exploration and Development, Beijing, China;School of Engineering Science, University of Chinese Academy of Sciences, Beijing, China;Institute of Porous Flow and Fluid Mechanics, University of Chinese Academy of Sciences, Beijing, China;State Key Laboratory of Enhanced Oil Recovery, PetroChina Research Institute of Petroleum Exploration and Development, Beijing, China;

关键词: co-culture;    biosurfactant;    biodegradation;    Bacillus subtilis;    Pseudomonas aeruginosa;   

DOI  :  10.3389/fmicb.2023.1132831

 received in 2022-12-28, accepted in 2023-04-21,  发布年份 2023

来源: Frontiers

【 摘 要 】

Microbial remediation has been regarded as one of the most promising decontamination techniques for crude oil pollution. However, there are few studies on the interaction of bacteria in the microbial community during bioremediation. The aim of this work was to research the promotion of defined co-culture of Bacillus subtilis SL and Pseudomonas aeruginosa WJ-1 for biodegradation of crude oil. After 7 days of incubation, the analysis of residual oil, saturated and aromatic fraction in the samples showed that the degradation efficiency of them was significantly improved. The degradation efficiency of crude oil was enhanced from 32.61% and 54.35% in individual culture to 63.05% by the defined co-culture of strains SL and WJ-1. Furthermore, it was found that the defined co-culture system represented relatively excellent performance in bacterial growth, cell surface hydrophobicity (CSH) and emulsification activity. These results indicated that the combination of Bacillus subtilis and Pseudomonas aeruginosa can effectively promote the degradation and utilization of crude oil, which may provide a new idea for the improvement of bioremediation strategies.GRAPHICAL ABSTRACT

【 授权许可】

Unknown   
Copyright © 2023 Wu, Xiu, Yu, Huang, Yi and Ma.