DNA methylation enzymes in the kidneys of male and female BTBR ob/ob mice

Frontiers in Endocrinology

Endocrinology

Mariana Charleaux de Ponte¹  Ana Paula Malavolta Luz¹  Beatriz Maria Veloso Pereira¹  Karina Thieme¹ 

[1] Laboratório de Bases Celulares e Moleculares da Fisiologia Renal, Departamento de Fisiologia e Biofísica, Instituto de Ciências Biomédicas, Universidade de Sao Paulo, Sao Paulo, SP, Brazil;

关键词: epigenetics;    DNMT;    TET;    chronic kidney disease;    obesity and diabetes;   

DOI  :  10.3389/fendo.2023.1167546

 received in 2023-02-16, accepted in 2023-03-23,  发布年份 2023

来源: Frontiers

【 摘 要 】

Diabetic kidney disease (DKD) is the leading cause of the end-stage renal disease. Recent studies have shown that epigenetic modifications contribute to alterations in gene expression and the development of DKD. This study aimed to show an expression profile of key DNA (de)methylation enzymes (DNMT, TET proteins) and their differences between sexes under obesity and diabetic condition. Male and female black and tan brachyury (BTBR) ob/ob mice and their corresponding wild-type littermates (BTBR WT) were studied until 16 weeks of age. Metabolic parameters, kidney morphophysiology and the expression of fibrotic markers and epigenetic enzymes were studied in whole kidney tissue or specifically in the glomerulus. The results showed sexual dimorphism in the development of metabolic disease and in kidney morphophysiology. Female mice have a different profile of DNMTs expression in both WT and obese/diabetic condition. Furthermore, metabolic condition negatively modulated the glomerular expression of TET1 and TET3 only in females. To our knowledge, this is the first study that shows a kidney profile of the expression of key (de)methylation enzymes, DNMTs and TETs, in the BTBR ob/ob experimental model of DKD and its association with sex. The knowledge of this epigenetic profile may help future research to understand the pathophysiology of DKD in males and females.

【 授权许可】

Unknown   
Copyright © 2023 Veloso Pereira, Charleaux de Ponte, Malavolta Luz and Thieme