Interferon regulatory factor 3 mediates effective antiviral responses to human coronavirus 229E and OC43 infection

Frontiers in Immunology

Immunology

Kaiwen Liu¹  Kensuke Hirasawa¹  Maria Licursi¹  Joseph K. Sampson Duncan¹  Danyang Xu¹  D. Lorne Tyrrell²  Michael A. Joyce²  Holly A. Saffran²  Jin Gohda³  Yasushi Kawaguchi⁴ 

[1] Division of BioMedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St. John’s, NL, Canada;Li Ka Shing Institute of Virology, University of Alberta, Edmonton, AB, Canada;Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, AB, Canada;Research Center for Asian Infectious Diseases, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan;Research Center for Asian Infectious Diseases, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan;Division of Molecular Virology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan;Department of Infectious Disease Control, International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan;

关键词: SARS-CoV2;    OC43;    229E;    innate immunity;    interferon;    IRF1;    IRF3;    IRF7;   

DOI  :  10.3389/fimmu.2023.930086

 received in 2022-04-27, accepted in 2023-03-27,  发布年份 2023

来源: Frontiers

【 摘 要 】

Interferon regulatory factors (IRFs) are key elements of antiviral innate responses that regulate the transcription of interferons (IFNs) and IFN-stimulated genes (ISGs). While the sensitivity of human coronaviruses to IFNs has been characterized, antiviral roles of IRFs during human coronavirus infection are not fully understood. Type I or II IFN treatment protected MRC5 cells from human coronavirus 229E infection, but not OC43. Cells infected with 229E or OC43 upregulated ISGs, indicating that antiviral transcription is not suppressed. Antiviral IRFs, IRF1, IRF3 and IRF7, were activated in cells infected with 229E, OC43 or severe acute respiratory syndrome-associated coronavirus 2 (SARS-CoV-2). RNAi knockdown and overexpression of IRFs demonstrated that IRF1 and IRF3 have antiviral properties against OC43, while IRF3 and IRF7 are effective in restricting 229E infection. IRF3 activation effectively promotes transcription of antiviral genes during OC43 or 229E infection. Our study suggests that IRFs may be effective antiviral regulators against human coronavirus infection.

【 授权许可】

Unknown   
Copyright © 2023 Duncan, Xu, Licursi, Joyce, Saffran, Liu, Gohda, Tyrrell, Kawaguchi and Hirasawa