Targeted transcriptomic analysis of pancreatic adenocarcinoma in EUS-FNA samples by NanoString technology

Frontiers in Molecular Biosciences

Molecular Biosciences

C. Sánchez-Montes¹  L. Pedrosa²  E. C. Vaquero³  I. K. Araujo⁴  O. Sendino⁴  G. Soy⁴  G. Fernández-Esparrach⁵  A. Ginès⁵  F. Ausania⁶  T. Saurí⁷  J. Maurel⁸  F. M. Espósito⁹  M. Cuatrecasas^1,10  F. M. Pérez^1,11  S. López^1,11  C. Montironi^1,12 

[1] Endoscopy Unit, Gastroenterology Department, ICMDM, Hospital Clínic, Barcelona, Spain;Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain;Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain;Centro de Investigación Biomédica en Red en Enfermedades Hepáticas y Digestivas (CIBEREHD), Barcelona, Spain;Gastroenterology Department, ICMDM, Hospital Clínic, Barcelona, Spain;Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain;Endoscopy Unit, Gastroenterology Department, ICMDM, Hospital Clínic, Barcelona, Spain;Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain;Endoscopy Unit, Gastroenterology Department, ICMDM, Hospital Clínic, Barcelona, Spain;Facultat de Medicina i Ciències de la Salut, University of Barcelona (UB), Barcelona, Spain;Centro de Investigación Biomédica en Red en Enfermedades Hepáticas y Digestivas (CIBEREHD), Barcelona, Spain;Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain;Facultat de Medicina i Ciències de la Salut, University of Barcelona (UB), Barcelona, Spain;Department of General and Digestive Surgery, ICMDM, Hospital Clínic, Barcelona, Spain;Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain;Facultat de Medicina i Ciències de la Salut, University of Barcelona (UB), Barcelona, Spain;Medical Oncology Department, Translational Genomics and Targeted Therapies in Solid Tumors, ICMHO, Hospital Clínic, Barcelona, Spain;Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain;Facultat de Medicina i Ciències de la Salut, University of Barcelona (UB), Barcelona, Spain;Medical Oncology Department, Translational Genomics and Targeted Therapies in Solid Tumors, ICMHO, Hospital Clínic, Barcelona, Spain;Centro de Investigación Biomédica en Red en Enfermedades Hepáticas y Digestivas (CIBEREHD), Barcelona, Spain;Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain;Medical Oncology Department, Translational Genomics and Targeted Therapies in Solid Tumors, ICMHO, Hospital Clínic, Barcelona, Spain;Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain;Pathology Department, Centre of Biomedical Diagnosis (CDB), Hospital Clínic, Barcelona, Spain;Facultat de Medicina i Ciències de la Salut, University of Barcelona (UB), Barcelona, Spain;Pathology Department, Centre of Biomedical Diagnosis (CDB), Hospital Clínic, Barcelona, Spain;Pathology Department, Centre of Biomedical Diagnosis (CDB), Hospital Clínic, Barcelona, Spain;Molecular Biology Core, CDB, Hospital Clinic, Barcelona, Spain;

关键词: pancreatic adenocarcinoma;    EUS-FNA;    NanoString technology;    tumour stroma;    transcriptomic analysis;   

DOI  :  10.3389/fmolb.2023.1161893

 received in 2023-02-08, accepted in 2023-04-24,  发布年份 2023

来源: Frontiers

【 摘 要 】

Background: Integration of transcriptomic testing into EUS-FNA samples is a growing need for precision oncology in pancreatic ductal adenocarcinoma (PDAC). The NanoString platform is suitable for transcriptome profiling in low yield RNA samples.Methods: Inclusion of patients that underwent EUS-FNA cytological diagnosis of pancreatic ductal adenocarcinoma using 19G and/or 22G needles and subsequent surgical resection. Formalin-fixed, paraffin-embedded (FFPE) cytological and surgical samples underwent RNA extraction and transcriptomic analysis using a custom 52-gene NanoString panel of stromal PDAC features. Cell type abundance was quantified in FFPE specimens and correlated.Results: 18 PDAC patients were included. Mean EUS-FNA passes was 2 + 0.7. All FFPE passed the RNA quality control for genomic analysis. Hierarchical clustering on the global gene expression data showed that genes were differentially expressed between EUS and surgical samples. A more enriched cancer-associated fibroblasts and epithelial-mesenchymal transition transcriptomic profile was observed across surgical specimens whereas immunological biomarkers were more represented in EUS-FNA samples. Cytological examination confirmed a scanty representation of CAF and more immunological cell abundance in cytological samples in comparison to surgical specimens.Conclusion: Targeted transcriptomic NanoString profiling of PDAC samples obtained by EUS-FNA is a feasible approach for pre-surgical molecular analysis although stromal CAF/EMT mRNA biomarkers are underrepresented.

【 授权许可】

Unknown   
Copyright © 2023 Pedrosa, Araujo, Cuatrecasas, Soy, López, Maurel, Sánchez-Montes, Montironi, Saurí, Sendino, Pérez, Ausania, Fernández-Esparrach, Espósito, Vaquero and Ginès.