Frontiers in Immunology | |
Phenotypic alteration of low-density granulocytes in people with pulmonary post-acute sequelae of SARS-CoV-2 infection | |
Immunology | |
Michelle D. Tallquist1  Gehan Devendra2  Vivek R. Nerurkar3  Sandra P. Chang3  Natalie Subia3  Boonyanudh Jiyarom4  Dominic C. Chow5  Cecilia M. Shikuma5  Juwon Park6  Logan S. Dean6  | |
[1] Center for Cardiovascular Research, John A. Burns School of Medicine, University of Hawai’i at Mānoa, Honolulu, HI, United States;Department of Pulmonary and Critical Care, Queen’s Medical Center, Honolulu, HI, United States;Department of Medicine, John A. Burns School of Medicine, University of Hawai’i at Manoa, Honolulu, HI, United States;Department of Tropical Medicine, Medical Microbiology, and Pharmacology, John A. Burns School Medicine, University of Hawai’i at Manoa, Honolulu, HI, United States;Hawaii Center for AIDS, John A. Burns School of Medicine, University of Hawai’i at Manoa, Honolulu, HI, United States;Hawaii Center for AIDS, John A. Burns School of Medicine, University of Hawai’i at Manoa, Honolulu, HI, United States;Department of Medicine, John A. Burns School of Medicine, University of Hawai’i at Manoa, Honolulu, HI, United States;Hawaii Center for AIDS, John A. Burns School of Medicine, University of Hawai’i at Manoa, Honolulu, HI, United States;Department of Tropical Medicine, Medical Microbiology, and Pharmacology, John A. Burns School Medicine, University of Hawai’i at Manoa, Honolulu, HI, United States; | |
关键词: SARS-CoV-2; long-COVID; post-acute sequalae of SARS-CoV-2 infection; pulmonary sequelae; low-density granulocyte; neutrophil extracellular traps; platelets; platelet-neutrophil aggregates; | |
DOI : 10.3389/fimmu.2022.1076724 | |
received in 2022-10-21, accepted in 2022-11-30, 发布年份 2022 | |
来源: Frontiers | |
【 摘 要 】
BackgroundLow-density granulocytes (LDGs) are a distinct subset of neutrophils whose increased abundance is associated with the severity of COVID-19. However, the long-term effects of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection on LDG levels and phenotypic alteration remain unexplored.MethodsUsing participants naïve to SARS-CoV-2 (NP), infected with SARS-CoV-2 with no residual symptoms (NRS), and infected with SARS-CoV-2 with chronic pulmonary symptoms (PPASC), we compared LDG levels and their phenotype by measuring the expression of markers for activation, maturation, and neutrophil extracellular trap (NET) formation using flow cytometry.ResultsThe number of LDGs was elevated in PPASC compared to NP. Individuals infected with SARS-CoV-2 (NRS and PPASC) demonstrated increased CD10+ and CD16hi subset counts of LDGs compared to NP group. Further characterization of LDGs demonstrated that LDGs from COVID-19 convalescents (PPASC and NRS) displayed increased markers of NET forming ability and aggregation with platelets compared to LDGs from NP, but no differences were observed between PPASC and NRS.ConclusionsOur data from a small cohort study demonstrates that mature neutrophils with a heightened activation phenotype remain in circulation long after initial SARS-CoV-2 infection. Persistent elevation of markers for neutrophil activation and NET formation on LDGs, as well as an enhanced proclivity for platelet-neutrophil aggregation (PNA) formation in COVID-19 convalescent individuals may be associated with PPASC prognosis and development.
【 授权许可】
Unknown
Copyright © 2022 Dean, Devendra, Jiyarom, Subia, Tallquist, Nerurkar, Chang, Chow, Shikuma and Park
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202310106935103ZK.pdf | 3062KB | download |