The role of thrombin in early-onset seizures

Frontiers in Cellular Neuroscience

Cellular Neuroscience

Alina Savotchenko¹  Dmytro Isaev¹  Mariia Klymenko¹  Mariia Shypshyna² 

[1]Department of Cellular Membranology, Bogomoletz Institute of Physiology, Kyiv, Ukraine

[2]Laboratory of Synaptic Transmission, Bogomoletz Institute of Physiology, Kyiv, Ukraine

关键词: thrombin;    blood-brain barrier;    hippocampus;    status epilepticus;    temporal-lobe epilepsy;    lithium-pilocarpine model;   

DOI  :  10.3389/fncel.2023.1101006

 received in 2022-11-17, accepted in 2023-02-20,  发布年份 2023

来源: Frontiers

【 摘 要 】

A variety of clinical observations and studies in animal models of temporal lobe epilepsy (TLE) reveal dysfunction of blood-brain barrier (BBB) during seizures. It is accompanied by shifts in ionic composition, imbalance in transmitters and metabolic products, extravasation of blood plasma proteins in the interstitial fluid, causing further abnormal neuronal activity. A significant amount of blood components capable of causing seizures get through the BBB due to its disruption. And only thrombin has been demonstrated to generate early-onset seizures. Using the whole-cell recordings from the single hippocampal neurons we recently showed the induction of epileptiform firing activity immediately after the addition of thrombin to the blood plasma ionic media. In the present work, we mimic some effects of BBB disruption in vitro to examine the effect of modified blood plasma artificial cerebrospinal fluid (ACSF) on the excitability of hippocampal neurons and the role of serum protein thrombin in seizure susceptibility. Comparative analysis of model conditions simulating BBB dysfunction was performed using the lithium-pilocarpine model of TLE, which most clearly reflects the BBB disruption in the acute stage. Our results demonstrate the particular role of thrombin in seizure-onset in conditions of BBB disruption.

【 授权许可】

Unknown   
Copyright © 2023 Savotchenko, Klymenko, Shypshyna and Isaev.