Based on disulfidptosis-related glycolytic genes to construct a signature for predicting prognosis and immune infiltration analysis of hepatocellular carcinoma

Frontiers in Immunology

Immunology

Zhijian Wang¹  Jiayi Peng²  Wenxiang Huang²  Jia Zhang²  Xuanxin Chen³  Xuenuo Chen³ 

[1] Department of General Practice, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China;Department of Geriatrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China;Department of Infectious Disease, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China;

关键词: hepatocellular carcinoma (HCC);    disulfidptosis;    glycolysis;    subtype;    prognostic signature;    tumor microenvironment;    SLCO1B1;   

DOI  :  10.3389/fimmu.2023.1204338

 received in 2023-04-12, accepted in 2023-08-04,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundHepatocellular carcinoma (HCC) comprises several distinct molecular subtypes with varying prognostic implications. However, a comprehensive analysis of a prognostic signature for HCC based on molecular subtypes related to disulfidptosis and glycolysis, as well as associated metabolomics and the immune microenvironment, is yet to be fully explored.MethodsBased on the differences in the expression of disulfide-related glycolytic genes (DRGGs), patients with HCC were divided into different subtypes by consensus clustering. Establish and verify a risk prognosis signature. Finally, the expression level of the key gene SLCO1B1 in the signature was evaluated using immunohistochemistry (IHC) and quantitative real-time PCR (qRT-PCR) in HCC. The association between this gene and immune cells was explored using multiplex immunofluorescence. The biological functions of the cell counting kit-8, wound healing, and colony formation assays were studied.ResultsDifferent subtypes of patients have specific clinicopathological features, prognosis and immune microenvironment. We identified seven valuable genes and constructed a risk-prognosis signature. Analysis of the risk score revealed that compared to the high-risk group, the low-risk group had a better prognosis, higher immune scores, and more abundant immune-related pathways, consistent with the tumor subtypes. Furthermore, IHC and qRT-PCR analyses showed decreased expression of SLCO1B1 in HCC tissues. Functional experiments revealed that SLCO1B1 overexpression inhibited the proliferation, migration, and invasion of HCC cells.ConclusionWe developed a prognostic signature that can assist clinicians in predicting the overall survival of patients with HCC and provides a reference value for targeted therapy.

【 授权许可】

Unknown   
Copyright © 2023 Wang, Chen, Zhang, Chen, Peng and Huang