A plasma proteomic approach in patients with heart failure after acute myocardial infarction: insights into the pathogenesis and progression of the disease

Frontiers in Cardiovascular Medicine

Cardiovascular Medicine

Da Huang¹  Tuan Cen¹  Baomin Wei¹  Hongying Wu¹  Xinshou Pan¹  LiuFang Zhou¹  Zhile Li¹  Yan Liu¹  Liuqing Wei¹  Liye Su²  Suren R. Sooranna³  ZhaoHe Huang⁴ 

[1] Department of Cardiology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China;Department of Cardiology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China;Graduate School, Youjiang Medical University for Nationalities, Baise, China;Department of Surgery and Cancer, Imperial College London, Chelsea and Westminster Hospital, London, United Kingdom;Life Science and Clinical Research Center, Youjiang Medical University for Nationalities, Baise, China;Graduate School, Youjiang Medical University for Nationalities, Baise, China;Affiliated Southwest Hospital, Youjiang Medical University for Nationalities, Baise, China;

关键词: acute myocardial infarction;    plasma proteomics;    heart failure;    disease progression;    biomarkers;   

DOI  :  10.3389/fcvm.2023.1153625

 received in 2023-01-29, accepted in 2023-04-27,  发布年份 2023

来源: Frontiers

【 摘 要 】

AimsThe pathogenesis of disease progression targets for patients with heart failure after acute myocardial infarction was investigated by using plasma proteomics.MethodsThe plasma proteomes of acute myocardial infarction patients with (MI-HF) and without (MI-WHF) heart failure were compared. Each group consisted of 10 patients who were matched for age and sex. The peptides were analyzed by 2-dimensional liquid chromatography coupled to tandem mass spectrometry in a high definition mode. Parallel reaction monitoring (PRM) verified the selected target proteins.ResultsWe identified and quantified 2,589 and 2,222 proteins, respectively, and found 117 differentially expressed proteins (DEPs) (≥1.5-fold), when the MI-HF and MI-WHF groups were compared. Of these 51 and 66 were significantly up-regulated and down-regulated, respectively. The significant DEPs was subjected to protein–protein interaction network analysis which revealed a central role of the NF-κB signaling pathway in the MI-HF patients. PRM verified that MB, DIAPH1, VNN1, GOT2, SLC4A1, CRP, CKM, SOD3, F7, DLD, PGAM2, GOT1, UBA7 and HYOU1 were 14 proteins which were highly expressed in MI-HF patients.ConclusionsThese findings showed a group of proteins related to the NF-κB signaling pathway in the pathogenesis of patients with poor outcomes after experiencing MI-HF. These proteins may be useful candidate markers for the diagnosis of MI-HF as well as help to elucidate the pathophysiology of this major cause of mortality in older patients.

【 授权许可】

Unknown   
© 2023 Liu, Huang, Li, Zhou, Cen, Wei, Wei, Wu, Su, Sooranna, Pan and Huang.