Application of transposon insertion site sequencing method in the exploration of gene function in microalgae

Frontiers in Microbiology

Microbiology

Hui Chen¹  Yulong Fan¹  Chengfeng Mao¹  Qiang Wang²  Xiaobing Hu³ 

[1] State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, China;State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, China;Academy for Advanced Interdisciplinary Studies, Henan University, Kaifeng, China;State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, China;School of Environmental Engineering, Yellow River Conservancy Technical Institute, Kaifeng, China;

关键词: microalgae;    chassis cell;    transposon;    flanking sequence;    sequencing;   

DOI  :  10.3389/fmicb.2023.1111794

 received in 2022-11-30, accepted in 2023-01-06,  发布年份 2023

来源: Frontiers

【 摘 要 】

Microalgae are a large group of organisms that can produce various useful substances through photosynthesis. Microalgae need to be genetically modified at the molecular level to become “Chassis Cells” for food, medicine, energy, and environmental protection and, consequently, obtain benefits from microalgae resources. Insertional mutagenesis of microalgae using transposons is a practical possibility for understanding the function of microalgae genes. Theoretical and technical support is provided in this manuscript for applying transposons to microalgae gene function by summarizing the sequencing method of transposon insertion sites.

【 授权许可】

Unknown   
Copyright © 2023 Hu, Fan, Mao, Chen and Wang.