yggS Encoding Pyridoxal 5′-Phosphate Binding Protein Is Required for Acidovorax citrulli Virulence

Frontiers in Microbiology

Microbiology

Yajie Liao¹  Yiyang Liu¹  Yanli Tian¹  Liming Xia¹  Lin Chen¹  Yuanjie Wang¹  Baohui Chen¹  Baishi Hu¹  Yuqiang Zhao²  Weirong Gong³ 

[1] College of Plant Protection and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing, China;Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-sen), Nanjing, China;Plant Protection and Quarantine Station of Province, Nanjing, China;

关键词: Aicdovorax citrulli;    virulence;    YggS;    type III secretion (T3S);    biological processes;   

DOI  :  10.3389/fmicb.2021.783862

 received in 2021-09-27, accepted in 2021-12-06,  发布年份 2022

来源: Frontiers

【 摘 要 】

Bacterial fruit blotch, caused by seed-borne pathogen Acidovorax citrulli, poses a serious threat to the production of cucurbits globally. Although the disease can cause substantial economic losses, limited information is available about the molecular mechanisms of virulence. This study identified that, a random transposon insertion mutant impaired in the ability to elicit a hypersensitive response on tobacco. The disrupted gene in this mutant was determined to be Aave_0638, which is predicted to encode a YggS family pyridoxal phosphate-dependent enzyme. YggS is a highly conserved protein among multiple organisms, and is responsible for maintaining the homeostasis of pyridoxal 5′-phosphate and amino acids in cells. yggS deletion mutant of A. citrulli strain XjL12 displayed attenuated virulence, delayed hypersensitive response, less tolerance to H2O2 and pyridoxine, increased sensitivity to antibiotic β-chloro-D-alanine, and reduced swimming. In addition, RNA-Seq analysis demonstrated that yggS was involved in regulating the expression of certain pathogenicity-associated genes related to secretion, motility, quorum sensing and oxidative stress response. Importantly, YggS significantly affected type III secretion system and its effectors in vitro. Collectively, our results suggest that YggS is indispensable for A.citrulli virulence and expands the role of YggS in the biological processes.

【 授权许可】

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Copyright © 2022 Wang, Zhao, Xia, Chen, Liao, Chen, Liu, Gong, Tian and Hu.