Group 1 and group 2 hemagglutinin stalk antibody response according to age

Frontiers in Immunology

Immunology

Peter Palese¹  Weina Sun¹  Teresa Aydillo²  Adolfo García-Sastre³  Iván Sanz-Muñoz⁴  Raúl Ortiz de Lejarazu⁴  Laura Sánchez-de Prada⁵  José María Eiros⁶ 

[1] Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, United States;Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, United States;Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY, United States;Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, United States;Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY, United States;Department of Pathology, Molecular and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, United States;Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, NY, United States;The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, United States;National Influenza Center of Valladolid, Valladolid, Spain;National Influenza Center of Valladolid, Valladolid, Spain;Department of Microbiology and Immunology, Hospital Clínico Universitario de Valladolid, Valladolid, Spain;National Influenza Center of Valladolid, Valladolid, Spain;Department of Microbiology and Immunology, Hospital Clínico Universitario de Valladolid, Valladolid, Spain;Centro de Investigación Biomédica en Red de Enfermedades Infecciosas, Instituto de Salud Carlos III, Madrid, Spain;

关键词: influenza;    stalk antibodies;    influenza vaccines;    age;    elderly;   

DOI  :  10.3389/fimmu.2023.1194073

 received in 2023-03-26, accepted in 2023-05-12,  发布年份 2023

来源: Frontiers

【 摘 要 】

ObjectiveAntibodies elicited by seasonal influenza vaccines mainly target the head of hemagglutinin (HA). However, antibodies against the stalk domain are cross-reactive and have been proven to play a role in reducing influenza disease severity. We investigated the induction of HA stalk-specific antibodies after seasonal influenza vaccination, considering the age of the cohorts.MethodsA total of 166 individuals were recruited during the 2018 influenza vaccine campaign (IVC) and divided into groups: <50 (n = 14), 50–64 (n = 34), 65–79 (n = 61), and ≥80 (n = 57) years old. Stalk-specific antibodies were quantified by ELISA at day 0 and day 28 using recombinant viruses (cH6/1 and cH14/3) containing an HA head domain (H6 or H14) from wild bird origin with a stalk domain from human H1 or H3, respectively. The geometric mean titer (GMT) and the fold rise (GMFR) were calculated, and differences were assessed using ANOVA adjusted by the false discovery rate (FDR) and the Wilcoxon tests (p <0.05).ResultsAll age groups elicited some level of increase in anti-stalk antibodies after receiving the influenza vaccine, except for the ≥80-year-old cohort. Additionally, <65-year-old vaccinees had higher group 1 antibody titers versus group 2 before and after vaccination. Similarly, vaccinees within the <50-year-old group showed a higher increase in anti-stalk antibody titers when compared to older individuals (≥80 years old), especially for group 1 anti-stalk antibodies.ConclusionSeasonal influenza vaccines can the induction of cross-reactive anti-stalk antibodies against group 1 and group 2 HAs. However, low responses were observed in older groups, highlighting the impact of immunosenescence in adequate humoral immune responses.

【 授权许可】

Unknown   
Copyright © 2023 Sánchez-de Prada, Sanz-Muñoz, Sun, Palese, Ortiz de Lejarazu, Eiros, García-Sastre and Aydillo