Microbiome analysis reveals potential for modulation of gut microbiota through polysaccharide-based prebiotic feeding in Oreochromis niloticus (Linnaeus, 1758)

Frontiers in Physiology

Physiology

Asit Kumar Bera¹  M. A. Hassan¹  Himanshu Sekhar Swain¹  Hemanta Chowdhury¹  Basanta Kumar Das¹  Nabanita Chakraborty¹  Ramesh Chandra Malick¹  Hirak Jyoti Chakraborty¹  Sandeep Ghatak² 

[1] Central Inland Fisheries Research Institute (ICAR), Bārākpur, India;The ICAR Research Complex for North Eastern Hill Region (ICAR RC NEH), Umiam, India;

关键词: Fish;    Oreochromis niloticus;    gut;    microbiome;    prebiotics;    plant polysaccharides;   

DOI  :  10.3389/fphys.2023.1168284

 received in 2023-02-17, accepted in 2023-05-23,  发布年份 2023

来源: Frontiers

【 摘 要 】

Characterization and functional profiling of the gut microbiota are essential for guiding nutritional interventions in fish and achieving favorable host-microbe interactions. Thus, we conducted a 30 days study to explore and document the gut microbial community of O. niloticus, as well as to evaluate the effects of a polysaccharide-based prebiotics with 0.5% and 0.75% Aloe vera extract on the gut microbiome through genomic analysis. The V3–V4 region of 16S rRNA was amplified and sequenced using Illumina HiSeq 2500, resulting in 1,000,199 reads for operational taxonomic unit (OTU) identification. Out of 8,894 OTUs, 1,181 were selected for further analysis. Our results revealed that Planctomycetes, Firmicutes, Proteobacteria, Verrucomicrobia, Actinobacteria, and Fusobacteria were the dominant phyla in both control and treatment samples. Higher doses of prebiotics were found to improve Planctomycetes and Firmicutes while decreasing Proteobacteria and Verrucomicrobia. We observed increasing trends in the abundance of Bacilli, Bacillaceae, and Bacillus bacteria at the class, family, and genus levels, respectively, in a dose-dependent manner. These findings were consistent with the conventional colony count data, which showed a higher prevalence of Bacillus in prebiotic-supplemented groups. Moreover, predicted functional analysis using PICRUSt indicated a dose-dependent upregulation in glycolysis V, superpathway of glycol metabolism and degradation, glucose and xylose degradation, glycolysis II, and sulfoglycolysis pathways. Most of the energy, protein, and amino acid synthesis pathways were upregulated only at lower doses of prebiotic treatment. Our findings suggest that the gut microbiome of O. niloticus can be optimized through nutritional interventions with plant-based polysaccharides for improved growth performance in commercial fish.

【 授权许可】

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Copyright © 2023 Bera, Chowdhury, Ghatak, Malick, Chakraborty, Chakraborty, Swain, Hassan and Das.