Loss of Hepatic Surf4 Depletes Lipid Droplets in the Adrenal Cortex but Does Not Impair Adrenal Hormone Production

Frontiers in Cardiovascular Medicine

Cardiovascular Medicine

Da-wei Zhang¹  Hong-mei Gu¹  Xiaole Chang²  Shucun Qin²  Yongfang Zhao²  Bingxiang Wang²  Lei Zhai²  Hao Wang²  Boyan Liu² 

[1] Department of Pediatrics and Group on the Molecular and Cell Biology of Lipids, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Canada;Institute of Atherosclerosis, College of Basic Medical Sciences, Shandong First Medical University, Shandong Academy of Medical Sciences, Tai'an, China;

关键词: proprotein convertase subtilisin/kexin 9;    LDL–cholesterol;    cholesterol;    triglyceride;    atherosclerosis;    LDL receptor (LDLR);   

DOI  :  10.3389/fcvm.2021.764024

 received in 2021-08-24, accepted in 2021-10-20,  发布年份 2021

来源: Frontiers

【 摘 要 】

The adrenal gland produces steroid hormones to play essential roles in regulating various physiological processes. Our previous studies showed that knockout of hepatic Surf4 (Surf4LKO) markedly reduced fasting plasma total cholesterol levels in adult mice, including low-density lipoprotein and high-density lipoprotein cholesterol. Here, we found that plasma cholesterol levels were also dramatically reduced in 4-week-old young mice and non-fasted adult mice. Circulating lipoprotein cholesterol is an important source of the substrate for the production of adrenal steroid hormones. Therefore, we investigated whether adrenal steroid hormone production was affected in Surf4LKO mice. We observed that lacking hepatic Surf4 essentially eliminated lipid droplets and significantly reduced cholesterol levels in the adrenal gland; however, plasma levels of aldosterone and corticosterone were comparable in Surf4LKO and the control mice under basal and stress conditions. Further analysis revealed that mRNA levels of genes encoding enzymes important for hormone synthesis were not altered, whereas the expression of scavenger receptor class B type I (SR-BI), low-density lipoprotein receptor (LDLR) and 3-hydroxy-3-methyl-glutaryl-CoA reductase was significantly increased in the adrenal gland of Surf4LKO mice, indicating increased de novo cholesterol biosynthesis and enhanced LDLR and SR-BI-mediated lipoprotein cholesterol uptake. We also observed that the nuclear form of SREBP2 was increased in the adrenal gland of Surf4LKO mice. Taken together, these findings indicate that the very low levels of circulating lipoprotein cholesterol in Surf4LKO mice cause a significant reduction in adrenal cholesterol levels but do not significantly affect adrenal steroid hormone production. Reduced adrenal cholesterol levels activate SREBP2 and thus increase the expression of genes involved in cholesterol biosynthesis, which increases de novo cholesterol synthesis to compensate for the loss of circulating lipoprotein-derived cholesterol in the adrenal gland of Surf4LKO mice.

【 授权许可】

Unknown   
Copyright © 2021 Chang, Zhao, Qin, Wang, Wang, Zhai, Liu, Gu and Zhang.