Biocontrol potential of Chitinophaga flava HK235 producing antifungal-related peptide chitinocin

Frontiers in Microbiology

Microbiology

Jae Woo Han¹  Jin Woo Lee²  Yeong Seok Kim²  Bomin Kim²  Hun Kim²  Gyung Ja Choi²  Da Yeon Kim³  Heung-Tae Kim⁴ 

[1] Center for Eco-Friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon, South Korea;Center for Eco-Friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon, South Korea;Department of Medicinal Chemistry and Pharmacology, University of Science and Technology, Daejeon, South Korea;Center for Eco-Friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon, South Korea;Department of Plant Medicine, Chungbuk National University, Cheongju, South Korea;Department of Plant Medicine, Chungbuk National University, Cheongju, South Korea;

关键词: Chitinophaga flava;    plant pathogen;    biocontrol;    antimicrobial peptide;    antifungal activity;   

DOI  :  10.3389/fmicb.2023.1170673

 received in 2023-02-21, accepted in 2023-05-05,  发布年份 2023

来源: Frontiers

【 摘 要 】

Botrytis cinerea is a necrotrophic fungal pathogen with an extremely broad host range, causing significant economic losses in agricultural production. In this study, we discovered a culture filtrate of bacterial strain HK235, which was identified as Chitinophaga flava, exhibiting high levels of antifungal activity against B. cinerea. From the HK235 culture filtrate, we isolated a new antimicrobial peptide molecule designated as chitinocin based on activity-guided fractionation followed by characterization of the amino acid composition and spectroscopic analyses. The HK235 culture filtrate and chitinocin completely inhibited both conidial germination and mycelial growth of B. cinerea at a concentration of 20% and 200 μg/mL, respectively. In addition to antibiosis against B. cinerea, the active compound chitinocin had a broad antifungal and antibacterial activity in vitro. When tomato plants were treated with the culture filtrate and chitinocin, the treatment strongly reduced the development of gray mold disease in a concentration-dependent manner compared to the untreated control. Here, considering the potent antifungal property in vitro and in vivo, we present the biocontrol potential of C. flava HK235 for the first time.

【 授权许可】

Unknown   
Copyright © 2023 Kim, Han, Lee, Kim, Kim, Kim, Choi and Kim.