Opportunities in optical and electrical single-cell technologies to study microbial ecosystems

Frontiers in Microbiology

Microbiology

Hira Khan¹  Valérie Mattelin¹  Nico Boon¹  Yuting Guo¹  Cristina García-Timermans¹  Josefien Van Landuyt¹  Fabian Mermans²  Filip Tavernier³  Ruben Van den Eeckhoudt⁴  Michael Kraft⁵  Irene Taurino⁶ 

[1] Center for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University, Ghent, Belgium;Center for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University, Ghent, Belgium;Department of Oral Health Sciences, KU Leuven, Leuven, Belgium;MICAS, Department of Electrical Engineering (ESAT), KU Leuven, Leuven, Belgium;Micro- and Nanosystems (MNS), Department of Electrical Engineering (ESAT), KU Leuven, Leuven, Belgium;Micro- and Nanosystems (MNS), Department of Electrical Engineering (ESAT), KU Leuven, Leuven, Belgium;Leuven Institute of Micro- and Nanoscale Integration (LIMNI), KU Leuven, Leuven, Belgium;Micro- and Nanosystems (MNS), Department of Electrical Engineering (ESAT), KU Leuven, Leuven, Belgium;Semiconductor Physics, Department of Physics and Astronomy, KU Leuven, Leuven, Belgium;

关键词: single-cell;    microbial ecology;    optical techniques;    electrical techniques;    flow cytometry;    Raman spectroscopy;    CMOS-MEA;    impedance flow cytometry;   

DOI  :  10.3389/fmicb.2023.1233705

 received in 2023-06-02, accepted in 2023-08-03,  发布年份 2023

来源: Frontiers

【 摘 要 】

New techniques are revolutionizing single-cell research, allowing us to study microbes at unprecedented scales and in unparalleled depth. This review highlights the state-of-the-art technologies in single-cell analysis in microbial ecology applications, with particular attention to both optical tools, i.e., specialized use of flow cytometry and Raman spectroscopy and emerging electrical techniques. The objectives of this review include showcasing the diversity of single-cell optical approaches for studying microbiological phenomena, highlighting successful applications in understanding microbial systems, discussing emerging techniques, and encouraging the combination of established and novel approaches to address research questions. The review aims to answer key questions such as how single-cell approaches have advanced our understanding of individual and interacting cells, how they have been used to study uncultured microbes, which new analysis tools will become widespread, and how they contribute to our knowledge of ecological interactions.

【 授权许可】

Unknown   
Copyright © 2023 Mermans, Mattelin, Van den Eeckhoudt, García-Timermans, Van Landuyt, Guo, Taurino, Tavernier, Kraft, Khan and Boon.

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