Unraveling the multifaceted resilience of arsenic resistant bacterium Deinococcus indicus

Frontiers in Microbiology

Microbiology

Sharon G. Wolf¹  Michael Elbaum²  Peter Kirchweger²  Dean O. Ranmar³  Ofra Golani³  Wilson D. T. Antunes⁴  Bruno A. Salgueiro⁵  Célia V. Romão⁵  André G. Gouveia⁵  Pedro M. Matias⁶ 

[1] Department of Chemical Research Support, Weizmann Institute of Science, Rehovot, Israel;Department of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot, Israel;Department of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel;Instituto Universitário Militar, Centro de Investigação da Academia Militar (CINAMIL), Unidade Militar Laboratorial de Defesa Biológica e Química (UMLDBQ), Lisbon, Portugal;Instituto de Tecnologia Química e Biológica António Xavier (ITQB NOVA), Universidade Nova de Lisboa, Oeiras, Portugal;Instituto de Tecnologia Química e Biológica António Xavier (ITQB NOVA), Universidade Nova de Lisboa, Oeiras, Portugal;Instituto de Biologia Experimental e Tecnológica (iBET), Oeiras, Portugal;

关键词: metals;    UV-C;    oxidative stress;    PolyP granules;    arsenate reductase;    arsenate;   

DOI  :  10.3389/fmicb.2023.1240798

 received in 2023-06-15, accepted in 2023-08-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

Arsenic (As) is a toxic heavy metal widely found in the environment that severely undermines the integrity of water resources. Bioremediation of toxic compounds is an appellative sustainable technology with a balanced cost-effective setup. To pave the way for the potential use of Deinococcus indicus, an arsenic resistant bacterium, as a platform for arsenic bioremediation, an extensive characterization of its resistance to cellular insults is paramount. A comparative analysis of D. indicus cells grown in two rich nutrient media conditions (M53 and TGY) revealed distinct resistance patterns when cells are subjected to stress via UV-C and methyl viologen (MV). Cells grown in M53 demonstrated higher resistance to both UV-C and MV. Moreover, cells grow to higher density upon exposure to 25 mM As(V) in M53 in comparison with TGY. This analysis is pivotal for the culture of microbial species in batch culture bioreactors for bioremediation purposes. We also demonstrate for the first time the presence of polyphosphate granules in D. indicus which are also found in a few Deinococcus species. To extend our analysis, we also characterized DiArsC2 (arsenate reductase) involved in arsenic detoxification and structurally determined different states, revealing the structural evidence for a catalytic cysteine triple redox system. These results contribute for our understanding into the D. indicus resistance mechanism against stress conditions.

【 授权许可】

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Copyright © 2023 Gouveia, Salgueiro, Ranmar, Antunes, Kirchweger, Golani, Wolf, Elbaum, Matias and Romão.