Biosynthesis and genetic engineering of phenazine-1-carboxylic acid in Pseudomonas chlororaphis Lzh-T5

Frontiers in Microbiology

Microbiology

Kaiquan Liu¹  Ruiming Wang¹  Piwu Li¹  Yanpeng Xu¹  Yufei Cao¹  Xiaoli Liang¹  Ling Li²  Zhenghua Li³ 

[1] State Key Laboratory of Biobased Material and Green Papermaking (LBMP), School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China;State Key Laboratory of Biobased Material and Green Papermaking (LBMP), School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China;Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China;State Key Laboratory of Biobased Material and Green Papermaking (LBMP), School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China;Shandong Provincial Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, China;

关键词: phenazine-1-carboxylic acid;    Pseudomonas chlororaphis;    genetic engineering;    non-scar deletion;    fed-batch fermentation;   

DOI  :  10.3389/fmicb.2023.1186052

 received in 2023-03-14, accepted in 2023-04-05,  发布年份 2023

来源: Frontiers

【 摘 要 】

Phenazine-1-carboxylic acid (PCA) is a biologically active substance with the ability to prevent and control crop diseases. It was certified as a pesticide by the Ministry of Agriculture of China in 2011 and was named “Shenzimycin.” Lzh-T5 is a Pseudomonas chlororaphis strain found in the rhizosphere of tomatoes. This strain can produce only 230 mg/L of PCA. We used LDA-4, which produces the phenazine synthetic intermediate trans-2,3-dihydro-3-hydroxyanthranilic acid in high amounts, as the starting strain. By restoring phzF and knocking out phzO, we achieved PCA accumulation. Moreover, PCA production was enhanced after knocking out negative regulators, enhancing the shikimate pathway, and performing fed-batch fermentation, thus resulting in the production of 10,653 mg/L of PCA. It suggested that P. chlororaphis Lzh-T5 has the potential to become an efficiency cell factory of biologically active substances.

【 授权许可】

Unknown   
Copyright © 2023 Liu, Li, Liang, Xu, Cao, Wang, Li and Li.