Transcriptome and proteome depth analysis indicate ABA, MAPK cascade and Ca2+ signaling co-regulate cold tolerance in Rhododendron chrysanthum Pall.

Frontiers in Plant Science

Plant Science

Yue Li¹  Hongwei Xu¹  Xiaofu Zhou¹  Kun Cao¹  Qingyi Zhang¹ 

[1] Key Laboratory of Plant Resource Science and Green Production of Jilin Province, Jilin Normal University, Siping, China;

关键词: cold stress;    Rhododendron chrysanthum;    proteome;    transcriptome;    abscisic acid;    MAPK cascade;    calcium ion;   

DOI  :  10.3389/fpls.2023.1146663

 received in 2023-01-17, accepted in 2023-02-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionCold stress is a global common problem that significantly limits plant development and geographical distribution. Plants respond to low temperature stress by evolving interrelated regulatory pathways to respond and adapt to their environment in a timely manner. Rhodoendron chrysanthum Pall. (R. chrysanthum) is a perennial evergreen dwarf shrub used for adornment and medicine that thrives in the Changbai Mountains at high elevations and subfreezing conditions.MethodsIn this study, a comprehensive investigation of cold tolerance (4°C, 12h) in R. chrysanthum leaves under cold using physiological combined with transcriptomic and proteomic approaches. ResultsThere were 12,261 differentially expressed genes (DEGs) and 360 differentially expressed proteins (DEPs) in the low temperature (LT) and normal treatment (Control). Integrated transcriptomic and proteomic analyses showed that MAPK cascade, ABA biosynthesis and signaling, plant-pathogen interaction, linoleic acid metabolism and glycerophospholipid metabolism were significantly enriched in response to cold stress of R. chrysanthum leaves.DiscussionWe analyzed the involvement of ABA biosynthesis and signaling, MAPK cascade, and Ca2+ signaling, that may jointly respond to stomatal closure, chlorophyll degradation, and ROS homeostasis under low temperature stress. These results propose an integrated regulatory network of ABA, MAPK cascade and Ca2+ signaling comodulating the cold stress in R. chrysanthum, which will provide some insights to elucidate the molecular mechanisms of cold tolerance in plants.

【 授权许可】

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Copyright © 2023 Zhang, Li, Cao, Xu and Zhou