Gut microbiome and mycobiome in inflammatory bowel disease patients with Clostridioides difficile infection

Frontiers in Cellular and Infection Microbiology

Cellular and Infection Microbiology

Yue Li¹  Si Yu¹  Yujie Shi¹  Bei Tan¹  Hui Xu¹  Yimin Dai¹  Jiaming Qian¹  Bowen Tian¹  Xiaomeng Ge²  Songnian Hu³ 

[1] Department of Gastroenterology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China;Microbial Resource and Big Data Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China;State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China;University of Chinese Academy of Sciences, Beijing, China;

关键词: inflammatory bowel disease;    Clostridioides difficile;    metagenomics;    gut microbiome;    mycobiome;   

DOI  :  10.3389/fcimb.2023.1129043

 received in 2022-12-21, accepted in 2023-01-24,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundClostridium difficile infection (CDI) is common in patients with inflammatory bowel disease (IBD) and has been reported as a risk factor for poor outcome. However, gut microbiome and mycobiome of IBD patients with CDI have been barely investigated. This study aimed to assess the gut microbiome and mycobiome in IBD patients with CDI.MethodsWe collected fecal samples from patients with active IBD and concomitant CDI (IBD-CDI group, n=25), patients with active IBD and no CDI (IBD-only group, n=51), and healthy subjects (HC, n=40). Patients’ characteristics including demographic data, disease severity, and medication history were collected. Metagenomic sequencing, taxonomic and functional analysis were carried out in the samples.ResultsWe found that the bacterial alpha diversity of the IBD-CDI group was decreased. The bacterial and fungal beta diversity variations between IBD patients and HC were significant, regardless of CDI status. But the IBD-CDI group did not significantly cluster separately from the IBD-only group. Several bacterial taxa, including Enterococcus faecium, Ruminococcus gnavus, and Clostridium innocuum were overrepresented in the IBD-CDI group. Furthermore, IBD patients with CDI were distinguished by several fungal taxa, including overrepresentation of Saccharomyces cerevisiae. We also identified functional differences in IBD patients with CDI include enrichment of peptidoglycan biosynthesis. The network analysis indicated specific interactions between microbial markers in IBD-CDI patients.ConclusionIBD patients with CDI had pronounced microbial dysbiosis. Gut micro-ecological changes in IBD patients with CDI might provide insight into the pathological process and potential strategies for diagnosis and treatment in this subset of patients.

【 授权许可】

Unknown   
Copyright © 2023 Yu, Ge, Xu, Tan, Tian, Shi, Dai, Li, Hu and Qian