Antifungal effects and biocontrol potential of lipopeptide-producing Streptomyces against banana Fusarium wilt fungus Fusarium oxysporum f. sp. cubense

Frontiers in Microbiology

Microbiology

Wenbo Han¹  Ruimei Wu¹  Xiaomin Yu¹  Yiping Zeng¹  Wenna Shan¹  Peifeng Xie¹  Shuang Guo²  Zhenghua Du³  Chanxin Chen³  Qianzhuo Mao⁴  Wei Wu⁴  Xiaxia Wang⁵  Zonghua Wang⁶ 

[1] FAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, China;FAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, China;College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, China;FAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, China;School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Plant Virology, Ningbo University, Ningbo, China;State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China;FAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, China;State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China;Fujian Universities Engineering Research Center of Marine Biology and Drugs, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou, China;

关键词: antifungal effect;    Streptomyces;    banana Fusarium wilt;    lipopeptide;    biocontrol;   

DOI  :  10.3389/fmicb.2023.1177393

 received in 2023-03-01, accepted in 2023-04-10,  发布年份 2023

来源: Frontiers

【 摘 要 】

Fusarium wilt of banana (FWB), caused by Fusarium oxysporum f. sp. cubense (Foc), especially tropical race 4 (TR4), presents the foremost menace to the global banana production. Extensive efforts have been made to search for efficient biological control agents for disease management. Our previous study showed that Streptomyces sp. XY006 exhibited a strong inhibitory activity against several phytopathogenic fungi, including F. oxysporum. Here, the corresponding antifungal metabolites were purified and determined to be two cyclic lipopeptide homologs, lipopeptin A and lipopeptin B. Combined treatment with lipopeptin complex antagonized Foc TR4 by inhibiting mycelial growth and conidial sporulation, suppressing the synthesis of ergosterol and fatty acids and lowering the production of fusaric acid. Electron microscopy observation showed that lipopeptide treatment induced a severe disruption of the plasma membrane, leading to cell leakage. Lipopeptin A displayed a more pronounced antifungal activity against Foc TR4 than lipopeptin B. In pot experiments, strain XY006 successfully colonized banana plantlets and suppressed the incidence of FWB, with a biocontrol efficacy of up to 87.7%. Additionally, XY006 fermentation culture application improved plant growth parameters and induced peroxidase activity in treated plantlets, suggesting a possible role in induced resistance. Our findings highlight the potential of strain XY006 as a biological agent for FWB, and further research is needed to enhance its efficacy and mode of action in planta.

【 授权许可】

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Copyright © 2023 Wang, Du, Chen, Guo, Mao, Wu, Wu, Han, Xie, Zeng, Shan, Wang and Yu.