Functional inference of long non-coding RNAs through exploration of highly conserved regions

Frontiers in Genetics

Genetics

Yanluo Zeng¹  Haiwen Liu¹  Zhongpeng Liu¹  Tianbin Guo¹  Hongyan Yin¹  Zhuoda Yin² 

[1] Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan University, Haikou, China;TJ-YZ School of Network Science, Haikou University of Economics, Haikou, China;

关键词: homolog clustering;    HCR;    XACT;    LINC00461;    functional inference;   

DOI  :  10.3389/fgene.2023.1177259

 received in 2023-03-01, accepted in 2023-04-28,  发布年份 2023

来源: Frontiers

【 摘 要 】

Background: Long non-coding RNAs (lncRNAs), which are generally less functionally characterized or less annotated, evolve more rapidly than mRNAs and substantially possess fewer sequence conservation patterns than protein-coding genes across divergent species. People assume that the functional inference could be conducted on the evolutionarily conserved long non-coding RNAs as they are most likely to be functional. In the past decades, substantial progress has been made in discussions on the evolutionary conservation of non-coding genomic regions from multiple perspectives. However, understanding their conservation and the functions associated with sequence conservation in relation to further corresponding phenotypic variability or disorders still remains incomplete.Results: Accordingly, we determined a highly conserved region (HCR) to verify the sequence conservation among long non-coding RNAs and systematically profiled homologous long non-coding RNA clusters in humans and mice based on the detection of highly conserved regions. Moreover, according to homolog clustering, we explored the potential function inference via highly conserved regions on representative long non-coding RNAs. On lncRNA XACT, we investigated the potential functional competence between XACT and lncRNA XIST by recruiting miRNA-29a, regulating the downstream target genes. In addition, on lncRNA LINC00461, we examined the interaction relationship between LINC00461 and SND1. This interaction or association may be perturbed during the progression of glioma. In addition, we have constructed a website with user-friendly web interfaces for searching, analyzing, and downloading to present the homologous clusters of humans and mice.Conclusion: Collectively, homolog clustering via the highly conserved region definition and detection on long non-coding RNAs, as well as the functional explorations on representative sequences in our research, would provide new evidence for the potential function of long non-coding RNAs. Our results on the remarkable roles of long non-coding RNAs would presumably provide a new theoretical basis and candidate diagnostic indicators for tumors.

【 授权许可】

Unknown   
Copyright © 2023 Liu, Guo, Yin, Zeng, Liu and Yin.