Rhizoma Atractylodis Macrocephalae—Assessing the influence of herbal processing methods and improved effects on functional dyspepsia

Frontiers in Pharmacology

Pharmacology

Jun-Mao Li¹  Qian-Feng Gong¹  Ling-Yun Zhong¹  Wen-Ting Wu¹  Yi Huang¹  Jing Zhu¹  Song-Hong Yang¹  Heng-Li Tong¹  Fei-Peng Gong² 

[1] School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang, China;School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang, China;Jiangxi Provincial People’s Hospital, The First Affiliated Hospital of Nanchang Medical College, Nanchang, China;

关键词: herbal processing method;    Rhizoma Atractylodis Macrocephalae;    network analysis;    functional dyspepsia;    ethnopharmacology;   

DOI  :  10.3389/fphar.2023.1236656

 received in 2023-06-08, accepted in 2023-07-24,  发布年份 2023

来源: Frontiers

【 摘 要 】

Background: The unique pharmaceutical methods for the processing of botanical drugs according to the theory of traditional Chinese medicine (TCM) affect clinical syndrome differentiation and treatment. The objective of this study was to comprehensively elucidate the principles and mechanisms of an herbal processing method by investigating the alterations in the metabolites of Rhizoma Atractylodis Macrocephalae (AMR) processed by Aurantii Fructus Immaturus (AFI) decoction and to determine how these changes enhance the efficacy of aqueous extracts in treating functional dyspepsia (FD).Methods: A qualitative analysis of AMR before and after processing was conducted using UPLC-Q-TOF-MS/MS, and HPLC was employed for quantitative analysis. A predictive analysis was then conducted using a network analysis strategy to establish a botanical drug–metabolite–target–disease (BMTD) network and a protein–protein interaction (PPI) network, and the predictions were validated using an FD rat model.Results: A total of 127 metabolites were identified in the processed AMR (PAMR), and substantial changes were observed in 8 metabolites of PAMR after processing, as revealed by the quantitative analysis. The enhanced aqueous extracts of processed AMR (PAMR) demonstrate improved efficacy in treating FD, which indicates that this processing method enhances the anti-inflammatory properties and promotes gastric motility by modulating DRD2, SCF, and c-kit. However, this enhancement comes at the cost of attenuating the regulation of motilin (MTL), gastrin (GAS), acetylcholine (Ach), and acetylcholinesterase (AchE).Conclusion: Through this series of investigations, we aimed to unravel the factors influencing the efficacy of this herbal formulation in improving FD in clinical settings.

【 授权许可】

Unknown   
Copyright © 2023 Yang, Zhu, Wu, Li, Tong, Huang, Gong, Gong and Zhong.