Nanofibrous insulin/vildagliptin core-shell PLGA scaffold promotes diabetic wound healing

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Shih-Jung Liu¹  Shu-Chun Huang²  Chen-Hung Lee³  Ming-Jer Hsieh³  Dong-Yi Chen³  Kuo-Chun Hung³  Chia-Jung Cho⁴ 

[1] Department of Orthopedic Surgery, Bone and Joint Research Center, Chang Gung Memorial Hospital-Linkou, Taoyuan, Taiwan;Department of Mechanical Engineering, Chang Gung University, Taoyuan, Taiwan;Department of Physical Medicine and Rehabilitation, New Taipei Municipal Tucheng Hospital, Chang Gung Memorial Hospital, New Taipei City, Taiwan;Department of Physical Medicine & Rehabilitation, Chang Gung Memorial Hospital, Linkou, Taiwan;College of Medicine, Chang Gung University, Taoyuan, Taiwan;Division of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital-Linkou, Chang Gung University College of Medicine, Taoyuan, Taiwan;Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung, Taiwan;

关键词: core-shell nanofiber;    diabetes;    wound;    insulin;    vildagliptin;   

DOI  :  10.3389/fbioe.2023.1075720

 received in 2022-10-20, accepted in 2023-04-11,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: Slow wound repair in diabetes is a serious adverse event that often results in loss of a limb or disability. An advanced and encouraging vehicle is wanted to enhance clinically applicable diabetic wound care. Nanofibrous insulin/vildagliptin core-shell biodegradable poly (lactic-co-glycolic acid) (PLGA) scaffolds to prolong the effective drug delivery of vildagliptin and insulin for the repair of diabetic wounds were prepared.Methods: To fabricate core-shell nanofibrous membranes, vildagliptin mixture with PLGA, and insulin solution were pumped via separate pumps into two differently sized capillary tubes that were coaxially electrospun.Results and Discussion: Nanofibrous core-shell scaffolds slowly released effective vildagliptin and insulin over 2 weeks in vitro migration assay and in vivo wound-healing models. Water contact angle (68.3 ± 8.5° vs. 121.4 ± 2.0°, p = 0.006) and peaked water absorbent capacity (376% ± 9% vs. 283% ± 24%, p = 0.003) of the insulin/vildagliptin core-shell nanofibrous membranes remarkably exceeded those of a control group. The insulin/vildagliptin-loaded core-shell nanofibers improved endothelial progenitor cells migration in vitro (762 ± 77 cells/mm2 vs. 424.4 ± 23 cells/mm2, p < 0.001), reduced the α-smooth muscle actin content in vivo (0.72 ± 0.23 vs. 2.07 ± 0.37, p < 0.001), and increased diabetic would recovery (1.9 ± 0.3 mm2 vs. 8.0 ± 1.4 mm2, p = 0.002). Core-shell insulin/vildagliptin-loaded nanofibers extend the drug delivery of insulin and vildagliptin and accelerate the repair of wounds associated with diabetes.

【 授权许可】

Unknown   
Copyright © 2023 Lee, Chen, Hsieh, Hung, Huang, Cho and Liu.