Integrating omics reveals that miRNA-guided genetic regulation on plant hormone level and defense response pathways shape resistance to Cladosporium fulvum in the tomato Cf-10-gene-carrying line

Frontiers in Genetics

Genetics

Fengjiao Liu¹  Dongye Zhang¹  Guan Liu²  Jingbin Jiang³  Huanhuan Yang³  Jingfu Li³  Xiangyang Xu³  He Zhang³  Tingting Zhao³ 

[1] College of Advanced Agriculture and Ecological Environment, Harbin, China;College of Advanced Agriculture and Ecological Environment, Harbin, China;College of Forestry, Harbin, China;College of Horticulture and Landscape Architecture, Harbin, China;

关键词: tomato;    Cladosporium fulvum;    Cf-10;    miRNA;    gene expression regulation;    defense response;   

DOI  :  10.3389/fgene.2023.1158631

 received in 2023-02-04, accepted in 2023-05-18,  发布年份 2023

来源: Frontiers

【 摘 要 】

Invasion of C. fulvum causes the most serious diseases affecting the reproduction of tomatoes. Cf-10-gene-carrying line showed remarkable resistance to Cladosporium fulvum. To exploit its defense response mechanism, we performed a multiple-omics profiling of Cf-10-gene-carrying line and a susceptible line without carrying any resistance genes at non-inoculation and 3 days post-inoculation (dpi) of C. fulvum. We detected 54 differentially expressed miRNAs (DE-miRNAs) between the non-inoculation and 3 dpi in the Cf-10-gene-carrying line, which potentially regulated plant-pathogen interaction pathways and hormone signaling pathways. We also revealed 3,016 differentially expressed genes (DEGs) between the non-inoculated and 3 dpi in the Cf-10-gene-carrying line whose functions enriched in pathways that were potentially regulated by the DE-miRNAs. Integrating DE-miRNAs, gene expression and plant-hormone metabolites indicated a regulation network where the downregulation of miRNAs at 3 dpi activated crucial resistance genes to trigger host hypersensitive cell death, improved hormone levels and upregulated the receptors/critical responsive transcription factors (TFs) of plant hormones, to shape immunity to the pathogen. Notably, our transcriptome, miRNA and hormone metabolites profiling and qPCR analysis suggested that that the downregulation of miR9472 potentially upregulated the expression of SAR Deficient 1 (SARD1), a key regulator for ICS1 (Isochorismate Synthase 1) induction and salicylic acid (SA) synthesis, to improve the level of SA in the Cf-10-gene-carrying line. Our results exploited potential regulatory network and new pathways underlying the resistance to C. fulvum in Cf-10-gene-carrying line, providing a more comprehensive genetic circuit and valuable gene targets for modulating resistance to the virus.

【 授权许可】

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Copyright © 2023 Liu, Liu, Zhang, Zhao, Yang, Jiang, Li, Zhang and Xu.