Multiple potential recombination events among Newcastle disease virus genomes in China between 1946 and 2020

Frontiers in Veterinary Science

Veterinary Science

Jia-Qi Zhao¹  Pir Tariq Shah²  Pei-Hua Wang²  Amina Nawal Bahoussi²  Yan-Yan Guo²  Li Xing³  Changxin Wu³ 

[1] Department of Bioengineering, College of Life Science, Shanxi University, Taiyuan, China;Institutes of Biomedical Sciences, Shanxi University, Taiyuan, China;Institutes of Biomedical Sciences, Shanxi University, Taiyuan, China;Shanxi Provincial Key Laboratory of Medical Molecular Cell Biology, Shanxi University, Taiyuan, China;Shanxi Provincial Key Laboratory for Prevention and Treatment of Major Infectious Diseases, Taiyuan, China;The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, China;

关键词: Newcastle disease virus;    recombination;    phylogeography;    oncolytic;    attenuated vaccine;   

DOI  :  10.3389/fvets.2023.1136855

 received in 2023-01-03, accepted in 2023-04-12,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionNewcastle Disease Virus (NDV) is a highly adaptable virus with large genetic diversity that has been widely studied for its oncolytic activities and potential as a vector vaccine. This study investigated the molecular characteristics of 517 complete NDV strains collected from 26 provinces across China between 1946-2020.MethodsHerein, phylogenetic, phylogeographic network, recombination, and amino acid variability analyses were performed to reveal the evolutionary characteristics of NDV in China.Results and discussionsPhylogenetic analysis revealed the existence of two major groups: GI, which comprises a single genotype Ib, and GII group encompassing eight genotypes (I, II, III, VI. VII. VIII, IX and XII). The Ib genotype is found to dominate China (34%), particularly South and East China, followed by VII (24%) and VI (22%). NDV strains from the two identified groups exhibited great dissimilarities at the nucleotide level of phosphoprotein (P), matrix protein (M), fusion protein (F), and haemagglutinin-neuraminidase (HN) genes. Consistently, the phylogeographic network analysis revealed two main Network Clusters linked to a possible ancestral node from Hunan (strain MH289846.1). Importantly, we identified 34 potential recombination events that involved mostly strains from VII and Ib genotypes. A recombinant of genotype XII isolated in 2019 seems to emerge newly in Southern China. Further, the vaccine strains are found to be highly involved in potential recombination. Therefore, since the influence of recombination on NDV virulence cannot be predicted, this report’s findings need to be considered for the security of NDV oncolytic application and the safety of NDV live attenuated vaccines.

【 授权许可】

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Copyright © 2023 Bahoussi, Shah, Zhao, Wang, Guo, Wu and Xing.