Assessment of lipid composition and eicosapentaenoic acid/docosahexaenoic acid bioavailability in fish oil obtained through different enrichment methods

Frontiers in Nutrition

Nutrition

Rongzhen Song¹  Wen Li¹  Yueliang Zhao¹  Ningping Tao²  Shanggui Deng³ 

[1]College of Food Science and Technology, Shanghai Ocean University, Shanghai, China

[2]College of Food Science and Technology, Shanghai Ocean University, Shanghai, China

[3]Shanghai Engineering Research Center of Aquatic-Product Processing and Preservation, Shanghai, China

[4]National Experimental Teaching Demonstration Center for Food Science and Engineering, Shanghai Ocean University, Shanghai, China

[5]College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang, China

关键词: eicosapentaenoic acid (EPA);    docosahexaenoic acid (DHA);    lipids composition;    Caco-2 cell;    bioavailability;   

DOI  :  10.3389/fnut.2023.1136490

 received in 2023-01-03, accepted in 2023-02-27,  发布年份 2023

来源: Frontiers

【 摘 要 】

In this study, we analyzed the eicosapentaenoic acid/docosahexaenoic acid (EPA/DHA) lipid composition of fish oil obtained through enzymatic treatment, fractional distillation and silica gel column purification, and further assessed EPA/DHA bioavailability. Lipid subclass composition information was obtained through ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS), and bioavailability tests were performed using the Caco-2 cell monolayer model. Results showed that enzymatic treatment improved the incorporation of EPA/DHA as diacylglycerol (DG) while silica gel column chromatography enriched the content of EPA/DHA as phosphatidylglycerol (PG) (12.58%) and phosphatidylethanolamine (PE) (4.99%). Furthermore, increasing the purity of EPA/DHA could improve its bioavailability and after 24 incubation, binding forms of triglyceride (TG) was superior to ethyl ester (EE) (p < 0.05) at the same purity level. Those findings are helpful to provide research basis for exploring the bioactivity of fish oil.

【 授权许可】

Unknown   
Copyright © 2023 Song, Li, Deng, Zhao and Tao.