期刊论文详细信息
Frontiers in Physiology
Optogenetic control of gut movements reveals peristaltic wave-mediated induction of cloacal contractions and reactivation of impaired gut motility
Physiology
Masafumi Inaba1  Shota Utsunomiya1  Yuuki Shikaya1  Ryosuke Tadokoro1  Yoshiko Takahashi2 
[1] Department of Zoology, Graduate School of Science, Kyoto University Kitashirakawa, Sakyo-ku, Kyoto;null;
关键词: optogeneitcs;    gut;    peristalsis;    chickens;    cloaca;   
DOI  :  10.3389/fphys.2023.1175951
 received in 2023-02-28, accepted in 2023-05-03,  发布年份 2023
来源: Frontiers
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【 摘 要 】

Gut peristalsis, recognized as a wave-like progression along the anterior-posterior gut axis, plays a pivotal role in the transportation, digestion, and absorption of ingested materials. The embryonic gut, which has not experienced ingested materials, undergoes peristalsis offering a powerful model for studying the intrinsic mechanisms underlying the gut motility. It has previously been shown in chicken embryos that acute contractions of the cloaca (an anus-like structure) located at the posterior end of the hindgut are tightly coupled with the arrival of hindgut-derived waves. To further scrutinize the interactions between hindgut and cloaca, we here developed an optogenetic method that produced artificial waves in the hindgut. A variant form of channelrhodopsin-2 (ChR2(D156C)), permitting extremely large photocurrents, was expressed in the muscle component of the hindgut of chicken embryos using Tol2-mediated gene transfer and in ovo electroporation techniques. The D156C-expressing hindgut responded efficiently to local pulses of blue light: local contractions emerge at an ectopic site in the hindgut, which were followed by peristaltic waves that reached to the endpoint of the hindgut. Markedly, the arrival of the optogenetically induced waves caused concomitant contractions of the cloaca, revealing that the hindgut-cloaca coordination is mediated by signals triggered by peristaltic waves. Moreover, a cloaca undergoing pharmacologically provoked aberrant contractions could respond to pulsed blue light irradiation. Together, the optogenetic technology developed in this study for inducing gut peristalsis paves the way to study the gut movement and also to explore therapeutic methodology for peristaltic disorders.

【 授权许可】

Unknown   
Copyright © 2023 Shikaya, Inaba, Tadokoro, Utsunomiya and Takahashi.

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