Whole blood transcriptome analysis in dairy calves experimentally challenged with bovine herpesvirus 1 (BoHV-1) and comparison to a bovine respiratory syncytial virus (BRSV) challenge

Frontiers in Genetics

Genetics

Bernadette Earley¹  Sinéad M. Waters¹  Dayle Johnston¹  Matthew S. McCabe¹  Stephanie O’Donoghue²  Derek W. Morris³  Jeremy F. Taylor⁴  Jae Woo Kim⁴  S. Louise Cosby⁵  Ken Lemon⁵  Catherine Duffy⁵  Michael McMenamy⁵ 

[1] Animal and Bioscience Research Department, Animal and Grassland Research and Innovation Centre, Teagasc, Grange, Meath, Ireland;Animal and Bioscience Research Department, Animal and Grassland Research and Innovation Centre, Teagasc, Grange, Meath, Ireland;Discipline of Biochemistry, National University of Ireland, Galway, Ireland;Discipline of Biochemistry, National University of Ireland, Galway, Ireland;Division of Animal Sciences, University of Missouri, Columbia, MO, United States;Veterinary Sciences Division, Agri-Food and Biosciences Institute, Stormont, Belfast, Northern Ireland;

关键词: BoHV-1;    transcriptome;    whole blood;    experimental challenge;    BRD;   

DOI  :  10.3389/fgene.2023.1092877

 received in 2022-11-08, accepted in 2023-01-25,  发布年份 2023

来源: Frontiers

【 摘 要 】

Bovine herpesvirus 1 (BoHV-1), is associated with several clinical syndromes in cattle, among which bovine respiratory disease (BRD) is of particular significance. Despite the importance of the disease, there is a lack of information on the molecular response to infection via experimental challenge with BoHV-1. The objective of this study was to investigate the whole-blood transcriptome of dairy calves experimentally challenged with BoHV-1. A secondary objective was to compare the gene expression results between two separate BRD pathogens using data from a similar challenge study with BRSV. Holstein-Friesian calves (mean age (SD) = 149.2 (23.8) days; mean weight (SD) = 174.6 (21.3) kg) were either administered BoHV-1 inoculate (1 × 107/mL × 8.5 mL) (n = 12) or were mock challenged with sterile phosphate buffered saline (n = 6). Clinical signs were recorded daily from day (d) −1 to d 6 (post-challenge), and whole blood was collected in Tempus RNA tubes on d six post-challenge for RNA-sequencing. There were 488 differentially expressed (DE) genes (p < 0.05, False Discovery rate (FDR) < 0.10, fold change ≥2) between the two treatments. Enriched KEGG pathways (p < 0.05, FDR <0.05); included Influenza A, Cytokine-cytokine receptor interaction and NOD-like receptor signalling. Significant gene ontology terms (p < 0.05, FDR <0.05) included defence response to virus and inflammatory response. Genes that are highly DE in key pathways are potential therapeutic targets for the treatment of BoHV-1 infection. A comparison to data from a similar study with BRSV identified both similarities and differences in the immune response to differing BRD pathogens.

【 授权许可】

Unknown   
Copyright © 2023 O’Donoghue, Earley, Johnston, McCabe, Kim, Taylor, Duffy, Lemon, McMenamy, Cosby, Morris and Waters.