Postnatal development of the relaxin-3 innervation of the rat medial septum

Frontiers in Neuroscience

Neuroscience

Andrew L. Gundlach¹  Mónica Navarro-Sánchez²  Isis Gil-Miravet²  Francisco Ros-Bernal²  Jorge Lucerón²  Esther Castillo-Gómez³  Francisco E. Olucha-Bordonau³ 

[1] The Florey Institute of Neuroscience and Mental Health, Florey Department of Neuroscience and Mental Health and Department of Anatomy and Physiology, The University of Melbourne, Parkville, VIC, Australia;Unitat Predepartamental de Medicina, Facultad de Ciencias de la Slud, Universitat Jaume I, Castellón, Spain;Unitat Predepartamental de Medicina, Facultad de Ciencias de la Slud, Universitat Jaume I, Castellón, Spain;Centro de Investigación Biomédica en Red de Salud Mental, (CIBERSAM), Madrid, Spain;

关键词: diagonal band;    neuropeptide;    nucleus incertus;    RXFP3;    septohippocampal system;    serotonin;    theta rhythm;   

DOI  :  10.3389/fnins.2023.1176587

 received in 2023-02-28, accepted in 2023-04-17,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionThe septal area provides a rich innervation to the hippocampus regulating hippocampal excitability to different behavioral states and modulating theta rhythmogenesis. However, little is known about the neurodevelopmental consequences of its alterations during postnatal development. The activity of the septohippocampal system is driven and/or modulated by ascending inputs, including those arising from the nucleus incertus (NI), many of which contain the neuropeptide, relaxin-3 (RLN3).MethodsWe examined at the molecular and cellular level the ontogeny of RLN3 innervation of the septal area in postnatal rat brains.ResultsUp until P13–15 there were only scattered fibers in the septal area, but a dense plexus had appeared by P17 that was extended and consolidated throughout the septal complex by P20. There was a decrease in the level of colocalization of RLN3 and synaptophysin between P15 and P20 that was reversed between P20 and adulthood. Biotinylated 3-kD dextran amine injections into the septum, revealed retrograde labeling present in the brainstem at P10-P13, but a decrease in anterograde fibers in the NI between P10–20. Simultaneously, a differentiation process began during P10–17, resulting in fewer NI neurons double-labeled for serotonin and RLN3.DiscussionThe onset of the RLN3 innervation of the septum complex between P17–20 is correlated with the onset of hippocampal theta rhythm and several learning processes associated with hippocampal function. Together, these data highlight the relevance and need for further analysis of this stage for normal and pathological septohippocampal development.

【 授权许可】

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Copyright © 2023 Ros-Bernal, Gil-Miravet, Lucerón, Navarro-Sánchez, Castillo-Gómez, Gundlach and Olucha-Bordonau.